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Elisa human il 10

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The ELISA (human IL-10) is a laboratory equipment used to detect and quantify the presence of human interleukin-10 (IL-10) in a sample. It is a type of enzyme-linked immunosorbent assay (ELISA) that employs antibodies and color change to measure the concentration of IL-10 in a sample.

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Phorbol myristate acetate (pma), by Enzo Life Sciences (1 mentions)

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IL-10 (167 citations) ELISAs (15 citations) Human IL-10 (7 citations) Human IL-10 ELISA MAX Deluxe kit (4 citations) ELISA Max Deluxe Set Human IL-10 (4 citations) ELISA MAX™ Standard Set Human IL-10 (3 citations) Human CXCL10 and IL-10 ELISA assays (2 citations) Human IL-10 ELISA MAX Standard kit (2 citations)

2 protocols using elisa human il 10

1

THP-1 Macrophage Activation Assay

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THP-1 cells were seeded at 5 × 105 cells/well in 24-well plate in complete medium with 100 ng/ml PMA (Enzo Life Sciences) during 48 h, for differentiation in M0-macrophages. After differentiation, adherent M0 were washed with 2% FBS medium and co-incubated with bacteria (MOI 50) or its control (PBS-glycerol) in 2% FBS medium for 24 h. IL-10 was quantified in supernatant by ELISA (human IL-10; BioLegend).
Relizani K., Le Corf K., Kropp C., Martin-Rosique R., Kissi D., Déjean G., Bruno L., Martinez C., Rawadi G., Elustondo F., Mazier W, & Claus S.P. (2022). Selection of a novel strain of Christensenella minuta as a future biotherapy for Crohn’s disease. Scientific Reports, 12, 6017.
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2

Assessing Bacterial-Induced IL-10 Secretion

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Assays were performed on three healthy donors. PBMCs were seeded at 1 million per well in 24-well plate. Bacteria were added at a MOI 50 or PBS-Glycerol, as a control, and co-cultures were maintained for 24 h. Interleukine-10 (IL-10) was quantified in supernatant by ELISA (human IL-10; BioLegend).
Relizani K., Le Corf K., Kropp C., Martin-Rosique R., Kissi D., Déjean G., Bruno L., Martinez C., Rawadi G., Elustondo F., Mazier W, & Claus S.P. (2022). Selection of a novel strain of Christensenella minuta as a future biotherapy for Crohn’s disease. Scientific Reports, 12, 6017.
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