The fat content was measured with
Ingenia 3.0 TMR scanner (Philips Healthcare), with a 32‐channel body coil, 6‐echo mDixon‐Quant gradient echo sequence, sagittal scan. The parameters were set up as follows: TR, 5.6 ms; TE, 0.98 ms; △TE, 0.7 ms; flip angle, 3°; voxel, 2.5 mm × 2.5 mm × 3 mm; matrix, 160 × 140; excitation frequency, one time; layer thickness, 3 mm; FOV, 400 mm × 350 mm × 231 mm; scanning time, 15 s. All data was transferred to the post‐processing workstation using
ISP software (version 7, Philips Healthcare), automatically generating fat score images for measurement. The most central image of the lumbar spine in the sagittal position was selected for the region of interest (ROI) on the L1‐L3 vertebral body. The ROI required the largest range to be included in the entire vertebral body as well as avoid the cortical bone, endplate and intervertebral disc. All measurements were performed independently by two residents, and the average value of the measurements was taken.
Zhu S., Tian A., Guo L., Xu H., Li X., Wang Z, & He F. (2021). Investigation of Diagnostic Biomarkers for Osteoporosis Based on Differentially Expressed Gene Profile with QCT and mDixon‐Quant Techniques. Orthopaedic Surgery, 13(7), 2137-2144.