Cyclic gmp competitive elisa kit

In order to evaluate the cGMP production [38 (link)] in HUVEC cells exposed to tested compounds (see Section 2.4), the cGMP content was measured by using the Cyclic GMP competitive ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA) following the manufacturer protocol.

In order to evaluate nitrite levels and cGMP production [32 (link)], HAEC cells were grown in 6-well plates and exposed to apocynin (100 µM), L-NNA (100 µM), the tested compounds (1 µM), or vehicle (MeOH) in phenol-free EBM-2 medium. After 24 h, the supernatants were collected and assayed for nitrite quantification by using the Nitrate/Nitrite Fluorometric Assay Kit (Cayman Chemicals, Ann Arbor, MI, USA). Afterwards, the cells were treated with 300 μL of HCl 0.1 μM and, after 15 min, were scraped and centrifuged in small Eppendorf tubes to collect supernatants. cGMP content was assessed using the Cyclic GMP competitive ELISA Kit (Cayman Chemical, MI, USA), following the manufacturer’s protocol.