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Anti human cd61 percp

Manufactured by BD
Sourced in United States

Anti-human CD61/PerCP is a monoclonal antibody used for the detection and identification of CD61-positive cells in flow cytometric analysis. The antibody is conjugated with the PerCP (Peridinin Chlorophyll Protein) fluorochrome, which allows for the detection of CD61-expressing cells. This product is intended for research use only and is not for use in diagnostic procedures.

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2 protocols using anti human cd61 percp

1

Quantifying Platelet Activation: Biochemical and Biophysical Analyses

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PSB 0777 (ammonium salt) was purchased from Tocris Bioscience (Bristol, United Kingdom) and cangrelor (sodium salt, AR-C69931MX) was from Cayman Chemical (Ann Arbor, MI, United States). Human serum albumin (HSA, purity 96–99%), warfarin sodium salt, ibuprofen sodium salt, and adenosine 5′-diphosphate (ADP, purity ≥95%), prostaglandin E1 (PGE1) were obtained from Sigma (St. Louis, MO, United States). Monoclonal antibodies anti-human CD61/PerCP (Cat# 347408, RRID: AB_2811174), CD62/PE (Cat# 348107, RRID: AB_2184974), mouse IgG1/PE isotype control (Cat# 340013, RRID: AB_399997) and Cellfix were obtained from Becton Dickinson (San Diego, CA, United States). Biacore sensor chips (CM5), Biacore amine coupling kit and BIAmaintenance kit were from GE Healthcare (Little Chalfont, United Kingdom). The BCA Protein Assay Kit was purchased from Thermo Scientific (Waltham, MA, United States). Phosphate buffered saline (PBS) was obtained from Biomed-Lublin (Lublin, Poland). All other chemicals, unless otherwise stated, were purchased from Avantor Performance Materials (Gliwice, Poland). All reagents were of analytical grade. Water used for solution preparation and glassware washing was passed through an Easy Pure UF water purification system (Thermolyne Barnstead, IA, United States).
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2

Platelet Function Assays Using Inhibitors

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Cangrelor (sodium salt, AR-C69931MX) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Prasugrel metabolite (R-138727) was obtained from BOC Sciences (Shirley, NY, USA). PSB 0739 (sodium salt), MRS 2279 (diammonium salt) and MRS 2179 (tetrasodium salt) were obtained from Tocris Bioscience (Bristol, UK). Monoclonal antibodies anti-human CD61/PerCP (Cat# 347408, RRID: AB_2811174), CD62P/PE (Cat# 348107, RRID: AB_2184974), mouse IgG1/PE isotype control (Cat# 340013, RRID: AB_399997), Cellfix, blood collection tubes containing 3.2% buffered sodium citrate solution were purchased from Becton-Dickinson (Franklin Lakes, NJ, USA). Adenosine 5 ′ -diphosphate (ADP, purity ≥95%) was purchased from Sigma (St. Louis, MO, USA). All other chemicals, unless otherwise stated, were purchased from Avantor Performance Materials (Gliwice, Poland). Water used for solution preparation and glassware washing was passed through an Easy Pure UF water purification system (Barnstead Thermolyne, Dubuque, IA, USA).
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