Sureprint g3 rat gene expression microarrays

RNA quality was assessed with Agilent 2100 Bioanalyzer (Agilent Technologies). RNA with RNA integrity number (RIN) > 7 was used for microarray analyses. Labelled cRNA was synthesized from 100 ng of total RNA using the Low Input Quick-Amp Labelling Kit, one colour (Agilent Technologies) in the presence of cyanine 3-CTP. Total RNA was hybridized on SurePrint G3 Rat Gene Expression Microarrays (#G4858A-074036, Agilent Technologies). This microarray consists of 60-mer DNA probes synthesized in situ, which represent 30,584 rat transcripts. Hybridization was performed at 65°C for 17 hours in a rotating oven (Microarray Facility, Laboratorio per le Tecnologie delle Terapie Avanzate, LTTA, Ferrara, Italy). One-color gene expression analysis was performed according to manufacturer's procedure. Feature Extraction 10.7.3 software (Agilent Technologies) was used to obtain microarray raw data. A fold change of ±1.5 and a FDR-adjusted p-value < 0.05 were considered to obtain the list of genes differentially expressed between the two experimental conditions. Datasets and raw data are publicly-available in GEO Profile (GEO ID: 200143876).

Total RNA was extracted by means of RNeasy Micro kit (Qiagen) following the manufacturer's protocol.
RNA quality was assessed with Agilent 2100 Bioanalyzer (Agilent Technologies). RNA with RNA integrity number (RIN) > 7 was used for microarray analysis. Labeled cRNA was synthesized from 100 ng of total RNA using the Low Input Quick-Amp Labeling Kit, one color (Agilent Technologies) in the presence of cyanine 3-CTP. The microarray hybridization was performed by the Microarray Facility, Laboratorio per le Tecnologie delle Terapie Avanzate (LTTA), Ferrara, Italy. Total RNA was hybridized on SurePrint G3 Rat Gene Expression Microarrays (#G4858A-074036, Agilent Technologies). This microarray consists of 60-mer DNA probes synthesized in situ, which represent 30,584 rat transcripts. Hybridization was performed at 65°C for 17 hours in a rotating oven. One-color gene expression analysis was performed according to manufacturer's procedure. Feature Extraction 10.7.3 software (Agilent Technologies) was used to obtain microarray raw-data.
A fold change of ±1.5 and a FDR-adjusted p-value < 0.05 were considered to obtain the list of genes differentially expressed between the two experimental conditions. Datasets and raw data are publicly-available in GEO Profile (GEO ID: 200143876) .