Ten microliters of the RT-LAMP reagent mixture was added to the bottom of a 0.5-mL PCR tube (Sarstedt, Germany), and 10 μL of the Cas12a reagent mixture was added inside the cap of the tube (see Fig. S5 in the supplemental material). Five microliters of TNA was added to the bottom of the tube and mixed with the RT-LAMP reagent by pipetting up and down. The tube was gently capped and put in a thermocycler (Eppendorf, Germany) without closing the lid, and the bottom of the tube was kept at the optimal temperature for 30 to 40 min for the RT-LAMP reaction. When the RT-LAMP reaction was completed, the tube was flicked with the wrist, and the Cas12a reagent mixture was mixed with the RT-LAMP amplicon. The tube was then incubated in the thermocycler at a constant temperature of 37°C for 10 min. Samples that showed green fluorescence under the excitation of a UV lamp were regarded as positive, and photos were taken using a smartphone. The fluorescence intensity was also measured using a Qubit 4 fluorometer (Invitrogen, USA). Samples that showed a fluorescence intensity above the cutoff value were regarded as positive.
0.5 ml pcr tube
Manufactured by Sarstedt
Sourced in Germany
The 0.5-mL PCR tube is a laboratory equipment designed for use in polymerase chain reaction (PCR) experiments. It is a small, transparent tube that can hold a sample volume of up to 0.5 milliliters. The tube is made of high-quality materials suitable for PCR applications.
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Lab products found in correlation
2 protocols using 0.5 ml pcr tube
1
RT-LAMP and Cas12a-Based Detection of Viral RNA
2
RT-LAMP and Cas12a-Based Detection of Viral RNA
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Ten microliters of the RT-LAMP reagent mixture was added to the bottom of a 0.5-mL PCR tube (Sarstedt, Germany), and 10 μL of the Cas12a reagent mixture was added inside the cap of the tube (see Fig. S5 in the supplemental material). Five microliters of TNA was added to the bottom of the tube and mixed with the RT-LAMP reagent by pipetting up and down. The tube was gently capped and put in a thermocycler (Eppendorf, Germany) without closing the lid, and the bottom of the tube was kept at the optimal temperature for 30 to 40 min for the RT-LAMP reaction. When the RT-LAMP reaction was completed, the tube was flicked with the wrist, and the Cas12a reagent mixture was mixed with the RT-LAMP amplicon. The tube was then incubated in the thermocycler at a constant temperature of 37°C for 10 min. Samples that showed green fluorescence under the excitation of a UV lamp were regarded as positive, and photos were taken using a smartphone. The fluorescence intensity was also measured using a Qubit 4 fluorometer (Invitrogen, USA). Samples that showed a fluorescence intensity above the cutoff value were regarded as positive.
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