Snap25-GCaMP6s (JAX#025111), Thy1-YFP-H (JAX#003782), and Cx3cr1-GFP (JAX#005582) mouse lines were purchased from The Jackson Laboratory and maintained on the C57BL/6J background. Mice were group-housed in the UCSC animal facility, with 12 h light-dark cycle and access to food and water ad libitum. Mice of both sexes were used in the study. All animal studies were performed in accordance with protocols approved by the Institutional Animal Care and Use Committee (IACUC) of UCSC.
Snap25 gcamp6s
Manufactured by Jackson ImmunoResearch
Snap25-GCaMP6s is a fluorescent calcium indicator protein designed for monitoring neuronal activity. It consists of a genetically encoded calcium-binding protein fused to the Snap-tag protein. This construct allows for specific labeling and tracking of neural cells expressing Snap25, a synaptic protein.
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Lab products found in correlation
2 protocols using snap25 gcamp6s
1
Fluorescent Mouse Lines for Neuroscience
2
Generation of Kcnt1 Y777H Knockin Mice
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The animals included in this study were housed and used in compliance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee at the University of Vermont. Experimental mice were generated by first mating the Kcnt1 Y777H knockin line 17 to the Snap25-GCaMP6s line (Jackson Labs Stock No: 025111). 33 (link) Double heterozygous offspring were then mated to generate mice carrying the Snap25-GCaMP6s construct that were either homozygous Y777H or WT at the Kcnt1 locus. Kcnt1 Y777H genotyping was performed as previously reported 17 and Snap25-GCaMP6s was genotyped following the protocol supplied by Jackson Labs.
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