On day 19 after tumor injection, tumor tissues were collected from each group. The frozen sections were fixed in cold acetone for 15 minutes and incubated with rabbit anti‐mouse PD‐L1 (eBioscience) or rabbit anti‐mouse PD‐1 (eBioscience) + rat anti‐mouse CD8 (eBioscience) for 1 hour. Slides were washed with TBST for 5 minutes and secondary Abs (Alexa Fluor 647‐labeled goat anti‐rabbit Abs and/or Alexa Fluor 488‐labeled goat anti‐rat Abs; eBioscience) were added and incubated for 30 minutes. Slides were washed with TBST for 10 minutes and mounted with a Vectashield DAPI containing kit (Sigma, Saint Louis, MO, USA). PD‐1+CD8+ T cells and expression of PD‐L1 in the TME were then observed through a fluorescence microscope (Eclipse E800; Nikon, Tokyo, Japan).
Alexa fluor 647 labeled goat anti rabbit abs
Manufactured by Thermo Fisher Scientific
Alexa Fluor 647-labeled goat anti-rabbit antibodies are secondary antibodies that bind to rabbit primary antibodies. They are conjugated with the Alexa Fluor 647 fluorescent dye, which can be detected using instruments that excite and detect the Alexa Fluor 647 fluorophore.
Automatically generated - may contain errors
2 protocols using alexa fluor 647 labeled goat anti rabbit abs
1
Tumor Immune Cell Phenotyping
2
Immunofluorescent Analysis of CD11b+LOX-1+ Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumor tissues were embedded in freeze embedding medium. The frozen sections were xed and incubated with anti-CD11b (eBioscience) and anti-LOX-1 antibodies (eBioscience) for 60 minutes. The secondary antibodies (Alexa Fluor 647-labeled goat anti-rabbit Abs and Alexa Fluor 488-labeled goat antirat Abs)(eBioscience) were then added and incubated for 30 minutes. The slides were mounted with a Vectashield DAPI-containing kit. Then CD11b + LOX-1 + cells in the tumor tissue were visualized with uorescence microscopy.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!