After transfection (72 h), Jurkat cells were stained with PE-conjugated anti-Annexin V and 7-AAD (Biolegend). After transfection (24 h), primary CD8+T cells were stimulated using anti-CD3/CD28 (3 μg/ml). After stimulation (48 h), CD8+T cells were stained with PE-cy7-conjugated anti-CD3, APC-cy7-conjugated anti-CD8, PE-conjugated anti-Annexin V, and 7-AAD (Biolegend). The cells were analyzed using a BD LSR II flow cytometer and the FlowJo software.
Yin L.B., Song C.B., Zheng J.F., Fu Y.J., Qian S., Jiang Y.J., Xu J.J., Ding H.B., Shang H, & Zhang Z.N. (2019). Elevated Expression of miR-19b Enhances CD8+ T Cell Function by Targeting PTEN in HIV Infected Long Term Non-progressors With Sustained Viral Suppression. Frontiers in Immunology, 9, 3140.
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