Dionex ics

Formation water samples for geochemical analyses were taken during field collection and upon arrival at the Texas laboratory. Analyses were performed by Intertek (Houston, TX, USA; Supplementary Material File S1). Concentrations of anions and organic acids were measured by ion chromatography using a Dionex ICS (Thermo Scientific, Waltham, MA, USA). Concentrations of Al, Ba, B, Cd, Ca, Cr, Cu, Fe, Pb, Li, Mg, Mn, Ni, P, K, Si, Na, Sr and Zn were determined using inductively coupled plasma spectroscopy.

A Dionex ICS (Thermo Scientific) was used to measure glucose released from glycogen. After glycogen hydrolysis, 10 μl samples were injected for analysis. Each sample was injected twice. An isocratic 10-min method with the high-pH mobile phase consisting of 98% buffer B (100 mM NaOH, pH 13.0) and 2% buffer D (1 M sodium acetate, 100 mM NaOH) was used along with a Dionex CarboPac PA10 (2 × 250 mm) anion-exchange column. A fresh glucose standard curve was prepared by serially diluting glucose (Sigma # G6918; 100 ml) to obtain concentrations between 4000 μM and 0 μM in 0.1 M sodium acetate (pH 4.8) supplemented with 0.5 mg/ml BSA. Glucose peak areas were manually integrated, and glucose concentrations were interpolated from the standard curve. Nonlinear regression analysis using the Michaelis–Menten equation were performed on Prism (GraphPad). One-way ANOVA with Fisher’s LSD as the post hoc test was performed using Prism (GraphPad) with an n = 7 (14 total measurements on the Dionex ICS) for precursor rhGAA, an n = 5 (10 total measurements) for processed rhGAA, an n = 3 (6 total measurements) for rhGAA–kif, and an n = 2 (4 total measurements) for all other samples.