The proliferation ability of BCa cells was detected by EdU assay using the EdU Cell Proliferation Kit with Alexa Fluor 594 (Beyotime, China). Cells were washed with PBS three times and incubated with complete medium with 10 µM EdU in a cell incubator for at least 2 hours. Then, the cells were washed with PBS to remove the EdU probe and culture medium, xed with 4% paraformaldehyde for 10 min at room temperature, and stained with DAPI for 5 min in the dark. The cells were observed under a positive uorescence microscope (Olympus, Tokyo, Japan) at 100x magni cation in ve random elds. The staining signals were captured, analyzed, and shown as fold changes compared with the control. The experiment was repeated three times.
Positive uorescence microscope
Manufactured by Olympus
Sourced in Japan
The Olympus Positive Fluorescence Microscope is a laboratory instrument designed to visualize and analyze fluorescently labeled specimens. It utilizes a light source, such as a mercury or xenon lamp, to excite fluorescent dyes within the sample, allowing for the observation of specific cellular structures or molecules. The microscope is equipped with specialized filters and optics to capture the emitted fluorescent signals, providing high-contrast images for scientific research and analysis.
Automatically generated - may contain errors
3 protocols using positive uorescence microscope
1
Proliferation Assay of Breast Cancer Cells
2
Immunohistochemical Analysis of CD31 Expression
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The slices were dewaxed and rehydrated. Endogenous peroxidase was inactivated with 10% hydrogen peroxide for 10 minutes. The antigen was recovered by pepsin at 37°C for 10 minutes. The sections were blocked in 5% BSA for 30 minutes and then mixed with 1:500 CD31 antibody (#ab28364; Abcam) and incubated at room temperature for 1 hour. After washing, the slices were incubated with the secondary antibody for 1 hour at room temperature, and the nuclei were stained with DAPI (Sigma-Aldrich) for 5 minutes and sealed with glycerol. The stained image was captured under a positive uorescence microscope (Olympus, Tokyo, Japan), and the proportion of CD31 + area in each photo was measured by ImageJ software. The experiment was repeated three times.
3
Proliferation Assay of Breast Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The proliferation ability of BCa cells was detected by EdU assay using the EdU Cell Proliferation Kit with Alexa Fluor 594 (Beyotime, China). Cells were washed with PBS three times and incubated with complete medium with 10 µM EdU in a cell incubator for at least 2 hours. Then, the cells were washed with PBS to remove the EdU probe and culture medium, xed with 4% paraformaldehyde for 10 min at room temperature, and stained with DAPI for 5 min in the dark. The cells were observed under a positive uorescence microscope (Olympus, Tokyo, Japan) at 100x magni cation in ve random elds. The staining signals were captured, analyzed, and shown as fold changes compared with the control. The experiment was repeated three times.
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