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Cd45 pe cy5 j33

Manufactured by Beckman Coulter
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The CD45 PE-Cy5 (J33) is a flow cytometry reagent used for the identification and enumeration of leukocytes. It is a monoclonal antibody conjugated with the fluorochrome PE-Cy5, which binds to the CD45 antigen expressed on the surface of all human leukocytes. This reagent is designed to be used as a tool for immunophenotyping and cell analysis in a laboratory setting.

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Lab products found in correlation

Cd19 pe hib19, by BD (2 mentions) Cd235a glycophorin gly a pe cy7, by Beckman Coulter (2 mentions) Fitc 11e4b 7 6, by Beckman Coulter (2 mentions) Igm bv510 g20 127, by BD (2 mentions) Cd38 pe cy5 ls198 4 3, by Takara Bio (2 mentions) Cd15 apc hi98, by BD (2 mentions) Cd31 pecam pe wm59, by BD (2 mentions) May grunwald and giemsa stains, by Merck Group (2 mentions)

2 protocols using cd45 pe cy5 j33

1

Multilineage Cell Identification by FACS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Five thousand to 10,000 FACS-sorted erythroid cells (CD235a/glycophorin (GLY)-A PE-Cy7 or FITC (11E4B-7-6; Coulter)), plasmacytoid lymphocytes (CD19 PE (HIB19; BD), IgM BV510 (G20-127; BD), CD38 PE-Cy5 (LS198-4-3; Clontech)), neutrophils (CD15 APC (HI98; BD), CD31/PECAM PE (WM59; BD) and CD45 PE-Cy5 (J33; Coulter)) were cytospun onto slides (500 r.p.m. for 10 min), air dried, and stained with May-Grunwald and Giemsa stains (both from Sigma), washed with water, air dried, and mounted, followed by examination by light microscopy.
Sugimura R., Jha D.K., Han A., Soria-Valles C., da Rocha E.L., Lu Y.F., Goettel J.A., Serrao E., Rowe R.G., Malleshaiah M., Wong I., Sousa P., Zhu T.N., Ditadi A., Keller G., Engelman A.N., Snapper S.B., Doulatov S, & Daley G.Q. (2017). Haematopoietic stem and progenitor cells from human pluripotent stem cells. Nature, 545(7655), 432-438.
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2

Multilineage Cell Identification by FACS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Five thousand to 10,000 FACS-sorted erythroid cells (CD235a/glycophorin (GLY)-A PE-Cy7 or FITC (11E4B-7-6; Coulter)), plasmacytoid lymphocytes (CD19 PE (HIB19; BD), IgM BV510 (G20-127; BD), CD38 PE-Cy5 (LS198-4-3; Clontech)), neutrophils (CD15 APC (HI98; BD), CD31/PECAM PE (WM59; BD) and CD45 PE-Cy5 (J33; Coulter)) were cytospun onto slides (500 r.p.m. for 10 min), air dried, and stained with May-Grunwald and Giemsa stains (both from Sigma), washed with water, air dried, and mounted, followed by examination by light microscopy.
Sugimura R., Jha D.K., Han A., Soria-Valles C., da Rocha E.L., Lu Y.F., Goettel J.A., Serrao E., Rowe R.G., Malleshaiah M., Wong I., Sousa P., Zhu T.N., Ditadi A., Keller G., Engelman A.N., Snapper S.B., Doulatov S, & Daley G.Q. (2017). Haematopoietic stem and progenitor cells from human pluripotent stem cells. Nature, 545(7655), 432-438.
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