Blood samples were collected during each session within 10 minutes of arrival and prior to each ultrasound. Samples were drawn from an antecubital vein into a dried, heparinized or EDTA tube, depending on the analysis being performed. Both tubes were immediately centrifuged for 10 minutes (3500 RPM). Because it was not possible to carry out all analyses on the same day using point-of-care technology, plasma and serum samples were frozen at −80°C within 20 minutes of blood collection and stored for later analyses of muscle injury markers and biochemical parameters (Table 1 )
Poch 100i automated hematology analyzer
Manufactured by Sysmex
Sourced in France, Japan
The PocH-100i Automated Hematology Analyzer is a compact, automated device designed for performing complete blood count (CBC) analysis. It uses flow cytometry technology to analyze a small sample of blood, providing accurate measurements of various blood cell parameters, including red blood cells, white blood cells, and platelets.
Automatically generated - may contain errors
Lab products found in correlation
Cobas 8000, by Roche (2 mentions)
Accutrend lactate analyzer, by Roche (1 mentions)
Nucleocounter nc 200, by ChemoMetec (1 mentions)
Fortessa, by BD (1 mentions)
Cryostor cs10, by Merck Group (1 mentions)
Vi cell xr, by Beckman Coulter (1 mentions)
Clinimacs pbs edta buffer, by Miltenyi Biotec (1 mentions)
Clinimacs plus instrument, by Miltenyi Biotec (1 mentions)
8 protocols using poch 100i automated hematology analyzer
1
Comprehensive Blood Biomarker Analysis Protocol
2
Hematological Analysis of Blood Samples
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Whole-blood aliquots were subjected to blood analysis, using an automated hematology blood counter (Sysmex pocH-100i Automated Hematology Analyzer). The parameters included glucose (mg/dL), hemoglobin (Hb, g/dL), erythrocytes (1 × 106/μL), leucocytes (1 × 103/μL), and platelet count (1 × 103/μL), which were performed to rule out any hematological abnormalities developed due to low folate consumption.
3
Thromboelastometric Analysis of Acidified Blood
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Blood was collected by venipuncture and CTI (0.1 mg mL À1 ) immediately added. CTI-treated blood was then split into two tubes, containing either 0.58% 3 M lactic acid or an equal volume of HEPES-buffered saline (HBS) (pH 7.4). The pH of both blood samples was measured using a CCA-TS blood gas analyzer (OPTI Medical, Roswell, GA, USA). For the six blood draws, the pH of the control group was 7.42 AE 0.03 and for the acidified group was 6.98 AE 0.04. The blood was then added to ROTEM cups (Tem Systems, Munich, Germany) containing either 5 pM TF or an equivalent volume of buffer. Viscoelastic measurements for each individual were performed in duplicate for each pH level at 37 °C. Blood was also drawn into citrate tubes and subjected to cell counts using the pocH-100i automated hematology analyzer (Sysmex, Lincolnshire, IL, USA). These data appear in Table S1.
4
Comprehensive Blood Analysis Protocol
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Blood samples were collected at each session within 10 min after arrival at each key point. Samples were drawn from an antecubital vein into a dried, heparinized or EDTA tube according to the analysis to be performed. Both tubes were immediately centrifuged for 10 min (3500 RPM). Since it was not possible to carry out all the analyses on the same day by point-of care technologies, plasma and serum were frozen at −80°C within 20 min after blood collection for later analysis of muscle injury markers and biochemical variables. The hematology parameters (hemoglobin, red blood cell, white blood cell) were directly analyzed by pocH-100i™automated hematology analyzer (Sysmex, Villepinte, France). Cobas 8000 (RocheDiagnostics, Manheim, Germany) were used to perform serial determinations for C-reactive protein (CRP), urinary creatinine, creatinine, calcium, chloride, potassium, sodium, and cholesterol. The osmolality and urinary osmolality were measured on ARKRAY OSMO STATION OM-6050 (Menarini, Florence, Italy).
5
Retrospective Study of POC Testing Integration
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This retrospective study was conducted at an approximately 850-bed academic medical center with outpatient clinics throughout the local region. Data were obtained from two of these clinic sites, each providing urgent and primary care. The Urgent Care clinics are intended to treat non-life-threatening emergencies such as mild infections, cough, and sore throat. More critical issues such as chest pain and possible stroke are referred to the emergency department at the main medical center campus. The clinic sites employ both the Abaxis Piccolo Xpress (“Piccolo”) chemistry analyzer and Sysmex pocH-100i Automated Hematology Analyzer (“pocHi”). Both sites underwent interfacing of POC testing devices with the institutional EHR in June 2019 as described in detail below. Clinic testing oversight is done jointly by the clinics and the Pathology department. The Pathology department provides central quality assurance and laboratory directorship. Two employees travel between clinic sites throughout the health system and assist with training and competency. The data were collected as part of a retrospective study approved by the Institutional Review Board (protocol #202010420) covering the period from July 1, 2017 to October 22, 2020. This study was carried out in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki).
6
Isolation and Cryopreservation of CD3+ T Cells
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CD3+ cells were purified from healthy donors’ Leukopaks received from Hemacare (Northridge, CA, USA). Upon reception, the leukapheresis material was analyzed using the Sysmex pocH-100i Automated Hematology Analyzer (Sysmex, Milton Keynes, UK), diluted with CliniMACS PBS/EDTA buffer (Miltenyi, Bisley, UK), supplemented with 0.5% human AB serum (Seralab, Hayward Heath, UK) and centrifuged at 300 × g for 15 min at room temperature to remove platelets. The CD3+ fraction was purified from the plasma on the CliniMACS Plus instrument (Miltenyi) using the CliniMACS CD4 and CD8 reagents (Miltenyi) anti-CD4 and anti-CD8 monoclonal antibodies conjugated to superparamagnetic iron dextran particles. The viability of the final CD3+ product was assessed using the Vi-CELL XR (Beckman Coulter, High Wycombe, UK) and Nucleocounter NC-200 (ChemoMetec, Allerod, Denmark). The number of CD45+ cells, CD3+ cells, CD4+ cells, and CD8+ cells was evaluated before and after the cell separation on the CliniMACS by flow cytometry on the Fortessa (BD Biosciences, San Jose, CA, USA). The acceptance criteria for the CD3+ cell purification were set as follows: (1) viability over 95% and (2) number of CD3+ cells over 95%. Following purification, a working cell bank of CD3+ cells was cryopreserved in CryoStor CS10 (Sigma, Gillingham, UK) until use.
7
Blood Collection and Complete Blood Count
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Blood was collected from all female participants in a 2.7ml Plastic Citrate Tube (catalog # 363083; BD Vacutainer®) and in a 2ml K2EDTA containing plastic tube (catalog # 367841; BD Vacutainer®). Among women of child bearing age, it was collected only in the inter-menstrual period. A complete blood count (CBC) of the collected samples was performed on Sysmex pocH-100i® Automated Hematology Analyzer (Sysmex Corporation, Kobe, Japan). The sample was then stored at 2-8°C until further analysis.
8
Haematological Analysis in Cohort Study
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Haematological analysis, including blood cell counts, was performed at the collection time point with Sysmex pocH-100i Automated Hematology Analyzer (Sysmex Co, Kobe, Japan).
Lymphocytes, neutrophils and overall count of basophils, monocytes and eosinophils (mixture) were available for N=527 participants from the methylomic profiling sample.
Lymphocytes, neutrophils and overall count of basophils, monocytes and eosinophils (mixture) were available for N=527 participants from the methylomic profiling sample.
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