Horseradish peroxidase conjugated secondary goat anti mouse antibody

Manufactured by Zymo Research

Horseradish peroxidase conjugated secondary goat anti-mouse antibody is a laboratory reagent used for the detection and quantification of mouse proteins in various immunoassays. It is a conjugate of a goat-derived secondary antibody that binds to mouse primary antibodies and the enzyme horseradish peroxidase, which can be used to generate a colorimetric or chemiluminescent signal for visualization and quantification.

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Lab products found in correlation

Western lightning chemiluminescence reagent plus kit, by PerkinElmer (2 mentions) Biotrace, by Bio-Rad (1 mentions) Semi dry trans blot apparatus, by Bio-Rad (1 mentions) Semi dry transblot, by Bio-Rad (1 mentions)

2 protocols using horseradish peroxidase conjugated secondary goat anti mouse antibody

Western Blot Protein Detection

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The protein samples were resolved by SDS-PAGE, and then transferred to a Bio-Trace nitrocellulose membranes at 15 V for 30 min using a Semi-Dry Trans-blot apparatus (Bio-Rad, Hercules, CA). The membrane containing protein samples were first probed with primary antibody against the His-tag or FLAG-tag (mouse), and then probed with the horseradish peroxidase conjugated secondary goat anti-mouse antibody (Zymed Laboratories, San Francisco, CA). Immunoreactive proteins were detected using the Western Lightning Chemiluminescence Reagent Plus Kit (Perkin-Elmer Life Sciences, Boston, MA).

Dou Y., Rutanhira H., Schormann N., Deivanayagam C, & Fletcher H.M. (2021). PG1659 functions as anti-sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83. Molecular oral microbiology, 36(1), 80-91.

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Purified Protein Western Blot Analysis

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The overexpressed and purified rPG1660 was analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and the proteins were then transferred to Bio-Trace nitrocellulose membranes at 15 V for 30 min using a Semi-Dry Trans-blot apparatus (Bio-Rad, Hercules, CA). The Western-blot was first probed with primary antibody (mouse) against the His-tag, and then probed with the horseradish peroxidase conjugated secondary goat anti-mouse antibody (Zymed Laboratories, San Francisco, CA). Immunoreactive proteins were detected using the Western Lightning Chemiluminescence Reagent Plus Kit (Perkin-Elmer Life Sciences, Boston, MA).

Dou Y., Rutanhira H., Chen X., Mishra A., Wang C, & Fletcher H.M. (2017). Role of extracytoplasmic function sigma factor PG1660 (RpoE) in the oxidative stress resistance regulatory network of Porphyromonas gingivalis. Molecular oral microbiology, 33(1), 89-104.

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