Huvec crl 1730

HUVEC (CRL-1730) and PC-12 (CRL-1721) cells were acquired from the American Type Culture Collection (Manassas, VA, USA). The HUVECs were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Life Technologies, Carlsbad, CA, USA). PC-12 cells were cultured in a RPMI-1640 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 5% FBS (Gibco, Life Technologies, Carlsbad, CA, USA) and 10% horse serum (Gibco, Life Technologies, Carlsbad, CA, USA). All cells were cultured in a humidified incubator (Thermo Fisher Scientific, Waltham, MA USA) with 95% air and 5% CO₂ at 37 °C.

The human umbilical vein endothelial cells (HUVEC, CRL-1730), human brain microvascular endothelial cells (HBMEC, CRL-3245), human aorta-vascular smooth muscle cells (HA-VSMC, CRL-1999), and human monocytes THP-1 (TIB-202) cell lines were purchased from the American Type Culture Collection (Manassas VA, USA); The oil red O solution was obtained from Beyotime Biotechnology (Shanghai, China); the shRNA lentivirus vector and the polybrene were obtained from the Cyagen Bioscience Inc., (Suzhou, China); IL-12, IL-6, and TNF-α ELISA kits were provided by Boster Biological Technology Co. Ltd. (Wuhan, China); Tissue Total cholesterol Assay Kit was from Applygen Technologies Inc. Beijing, China); PrimeScript RT reagent Kit, SYBR Premix DimerEraser™ (Perfect Real Time) assay kit, and the primers were purchased from Takara (Dalian, China); the primary antibodies p-TNK1 (D46E7), TNK1 (C44F9), p-STAT1 (58D6), STAT1 (D1K9Y), p-Tyk2 (Y1054), and Tyk2 (9312S) were purchased from CST (Danvers, MA, USA); the primary antibody for β-actin (AP0060) was purchased from Bioworld Technology (Nanjing, China); and the oxLDL was provided by the Peking Union-Biology, Co. Ltd (Beijing, China).

Human gastric cancer cell lines AGS (CRL-1739), SGC-7901, MKN-28 and MKN-45, human primary stomach epithelial HPSEC cells (H-6039), SV40-immortalized normal gastric epithelial GES-1 cells and human endothelial cell line HUVEC (CRL-1730) were purchased from the American Type Culture Collection (Rockville, MD, USA), Cell Biologics Inc. (Chicago, IL, USA) and Type Culture Collection of Chinese Academy of Sciences (Shanghai, China), authenticated by the supplier, and used within 6 months after resuscitation of frozen aliquots. Cell lines were grown at 37 °C in a humidified atmosphere of 5% CO₂, with RPMI1640 medium (Life Technologies, Inc., Gaithersburg, MD, USA) or M6621 medium (Cell Biologics Inc.) containing 10% fetal bovine serum (Life Technologies, Inc.), penicillin (100 U/ml) and streptomycin (100 μg/ml).