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Human cxcl5 quantikine elisa kit

Manufactured by R&D Systems
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The Human CXCL5 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human CXCL5 levels in cell culture supernates, serum, and plasma.

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Human CXCL5/ENA-78 Quantikine ELISA Kit Human CXCL5 Quantikine ELISA kit Quantikine ELISA Quantikine Human CXCL5 Quantikine kits Quantikines Human kits ELISAs

3 protocols using human cxcl5 quantikine elisa kit

1

Quantifying CXCL5 Secretion by MSCs

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MSCs (2.5 × 104/cm2) were seeded into 12-plate wells and supernatants of MSCs were harvested on day 3. The level of CXCL5 protein in the cell culture supernatant was quantitated using Human CXCL5 Quantikine ELISA Kit (R&D Systems) following the manufacturer’s protocol.
Liu W., Wang P., Xie Z., Wang S., Ma M., Li J., Li M., Cen S., Tang S., Zheng G., Ye G., Wu X., Wu Y, & Shen H. (2019). Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis. Cell Death & Disease, 10(3), 188.
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2

HBMEC IL-17 Cytokine Stimulation

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Two days after seeding, HBMECs were stimulated with culture medium containing 250 ng/mL of mouse IL-17A, B, C, D, or E (R&D Systems, Inc.). Conditioned media from triplicate culture conditions was collected after 48 hours and human CXCL5 levels measured using a human CXCL5 Quantikine Elisa Kit (R&D Systems, Inc.). Absorbance values measured at 450 nm and absorbance at 570 nm was used for background subtraction. Background subtracted absorbance values were converted to pg/mL concentrations based on standard curve concentrations.
Xiao G., Kumar R., Komuro Y., Burguet J., Kakarla V., Azizkhanian I., Sheth S.A., Williams C.K., Zhang X.R., Macknicki M., Brumm A., Kawaguchi R., Mai P., Kaneko N., Vinters H.V., Carmichael S.T., Havton L.A., DeCarli C, & Hinman J.D. (2022). IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury. Cell reports, 41(12), 111848.
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3

CXCL5 Secretion in Pancreatic Cancer Cells

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AsPC-1 and BxPC-3 cells were treated with TSZ ± nec-1 or with DMSO (control) for 3 hours. Cells were then washed twice with PBS and placed in serum-free medium. After 12 hours’ incubation, CM was collected, and CXCL5 was quantified using a Human CXCL5 Quantikine ELISA Kit (R & D Systems Europe Ltd, Oxford, UK).
Ando Y., Ohuchida K., Otsubo Y., Kibe S., Takesue S., Abe T., Iwamoto C., Shindo K., Moriyama T., Nakata K., Miyasaka Y., Ohtsuka T., Oda Y, & Nakamura M. (2020). Necroptosis in pancreatic cancer promotes cancer cell migration and invasion by release of CXCL5. PLoS ONE, 15(1), e0228015.
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