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Tnfa alexafluor700

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The TNFa-AlexaFluor700 is a fluorescently labeled antibody that binds to the cytokine Tumor Necrosis Factor alpha (TNFα). It is designed for use in flow cytometry and other immunoassay applications to detect and quantify the presence of TNFα in biological samples.

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Lab products found in correlation

Efluor 780, by Thermo Fisher Scientific (2 mentions) Gata 3 pe, by Thermo Fisher Scientific (2 mentions) Ifng fitc, by Thermo Fisher Scientific (2 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (2 mentions) Il 4 apc, by BD (2 mentions) Il 4 apc, by Thermo Fisher Scientific (2 mentions) Ebio13a, by Thermo Fisher Scientific (2 mentions) Ebio17b7, by BD (2 mentions) Il 17f pe, by BD (2 mentions) Il 5 pe, by BioLegend (2 mentions) Il 13 fitc, by Thermo Fisher Scientific (2 mentions) Pvm13 1, by BD (2 mentions) Il 17a efluor450, by Thermo Fisher Scientific (2 mentions) Il 13 pe, by Thermo Fisher Scientific (2 mentions) Facsdiva 6, by BD (1 mentions) Lsr fortessa 2, by BD (1 mentions) Annexin 5 apc, by Immunotools (1 mentions) Cd45ra pe cy7, by BD (1 mentions) Fasl pe, by Thermo Fisher Scientific (1 mentions) Ccr7 apcefluor 780, by Thermo Fisher Scientific (1 mentions)

3 protocols using tnfa alexafluor700

1

Multiparametric Flow Cytometry for Cytokine and Transcription Factor Analysis

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For cytokine staining, cells were restimulated with 20 nM PMA and 1μM ionomycin for 4 hours, and 5μg/ml brefeldin A was added during the last 2 hours of restimulation. Cells were stained with the viability dye eFluor780 (eBiosciences; Cat # 65-0865-14), and then fixed in 4% paraformaldehyde for 8 minutes at room temperature. Cytokine staining was done in permeabilization buffer containing 0.05% saponin. For transcription factor staining, unstimulated cells were fixed, permeabilized, and stained using the FoxP3 Staining Kit (eBiosciences; Cat # 00-5523-00). Samples were analyzed with a flow cytometer (BD LSR II). Mouse antibodies: IL-13-PE (eBiosciences; eBio13A), IL-4-APC (eBiosciences; 11B11), IL-5-PE (Biolegend; TRFK5), IFNg-FITC (eBiosciences; XMG1.2), TNFa-AlexaFluor700 (BD Biosciences; MP6-XT22), and GATA-3-PE (eBiosciences; E50-2440). Human antibodies: IL-13-FITC (eBiosciences; PVM13-1), IL-4-APC (BD Biosciences; 8D4-8), IL-17A-eFluor450 (eBiosciences; eBio17B7), and IL-17F-PE (BD Pharmingen; 079-289).
Simpson L.J., Patel S., Bhakta N.R., Choy D.F., Brightbill H.D., Ren X., Wang Y., Pua H.H., Baumjohann D., Montoya M.M., Panduro M., Remedios K.A., Huang X., Fahy J.V., Arron J.R., Woodruff P.G, & Ansel. K.M. (2014). A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production. Nature immunology, 15(12), 1162-1170.
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2

Multiparametric Flow Cytometry for Cytokine and Transcription Factor Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cytokine staining, cells were restimulated with 20 nM PMA and 1μM ionomycin for 4 hours, and 5μg/ml brefeldin A was added during the last 2 hours of restimulation. Cells were stained with the viability dye eFluor780 (eBiosciences; Cat # 65-0865-14), and then fixed in 4% paraformaldehyde for 8 minutes at room temperature. Cytokine staining was done in permeabilization buffer containing 0.05% saponin. For transcription factor staining, unstimulated cells were fixed, permeabilized, and stained using the FoxP3 Staining Kit (eBiosciences; Cat # 00-5523-00). Samples were analyzed with a flow cytometer (BD LSR II). Mouse antibodies: IL-13-PE (eBiosciences; eBio13A), IL-4-APC (eBiosciences; 11B11), IL-5-PE (Biolegend; TRFK5), IFNg-FITC (eBiosciences; XMG1.2), TNFa-AlexaFluor700 (BD Biosciences; MP6-XT22), and GATA-3-PE (eBiosciences; E50-2440). Human antibodies: IL-13-FITC (eBiosciences; PVM13-1), IL-4-APC (BD Biosciences; 8D4-8), IL-17A-eFluor450 (eBiosciences; eBio17B7), and IL-17F-PE (BD Pharmingen; 079-289).
Simpson L.J., Patel S., Bhakta N.R., Choy D.F., Brightbill H.D., Ren X., Wang Y., Pua H.H., Baumjohann D., Montoya M.M., Panduro M., Remedios K.A., Huang X., Fahy J.V., Arron J.R., Woodruff P.G, & Ansel. K.M. (2014). A miRNA upregulated in asthma airway T cells promotes TH2 cytokine production. Nature immunology, 15(12), 1162-1170.
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3

Multiparametric Flow Cytometry of Immune Cells

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For cell surface markers, cells were stained in PBS containing 2% fetal bovine serum for 15 minutes, unless mentioned otherwise. For intracellular cytokine staining, cells were stimulated with phorbol 12-myristate acetate (PMA) (50 ng/ mL) and ionomycin (1 lg/mL), or with different antigens, the last 8 hours with 10 lg/mL Brefeldin A and/or 2 lmol/mL monensin, at 378C and 5% CO 2 as mentioned above, and then fixed and stained according to manufacturer's protocols. Antibodies used were as follows: CD3-Alexa Fluor 700, CD4-PerCP/Cy5.5A, IFN-c Alexa Fluor 488, IL-17-PerCP/Cy5.5A, CCR7-APCeFluor780 (eBioscience, San Diego, CA, USA), CD8-V500A (Tonbo, San Diego, CA, USA), TNF-a-Alexa Fluor 700(BD), CD45RA-PECy7, CD45RO-PECF594, FAS-PECF594 (BD, San Diego, CA, USA), FasL-PE (Invitrogen), AnnexinV-APC (Immuno Tools, GmbH, Germany). Cells were acquired and analyzed by BD LSR Fortessa II (BD), using FlowJo (Ashland, OR, USA) three-star or FACSDIVA 6 software (BD Biosciences, San Jose, CA, USA).
Tagirasa R., Parmar S., Barik M.R., Devadas S, & Basu S. (2017). Autoreactive T Cells in Immunopathogenesis of TB-Associated Uveitis. Investigative ophthalmology & visual science, 58(13).
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