Gr5a gal4

Flies were kept in vials containing a standard medium made of yeast, corn, and agar at 25°C, 60% relative humidity, and on a 12 hr light-12-hr dark cycle. Virgin female flies were collected shortly after eclosion and kept in groups (25 flies per vial) on standard fly medium (ND, with 10% sucrose) or HSD (ND plus an additional 10% sucrose) for 4–6 days before experiments.
Fly strains used in the manuscript: nosNGT-GAL4: (#31777), Maternal-tubulin-Gal4 (#2318), Gr5a-GAL4 (#57592), and UAS-GCaMP6m (#42748) were obtained from the Bloomington Drosophila Stock Center at Indiana University; UAS-E(z) RNAi (#2831), UAS-Pcl RNAi (#1185), UAS-Su(var)3–9 RNAi (#3558), UAS-Rpd3 RNAi (#0695), and UAS-cad RNAi (#03,877.N) were from the Tsinghua Fly Center.

Flies were kept in vials containing a standard medium made of yeast, corn, and agar at 25°C, 60% relative humidity, and on a 12 hr light-12-hr dark cycle. Virgin female flies were collected shortly after eclosion and kept in groups (25 flies per vial) on standard fly medium (ND, with 10% sucrose) or HSD (ND plus an additional 10% sucrose) for 4–6 days before experiments.
Fly strains used in the manuscript: nosNGT-GAL4: (#31777), Maternal-tubulin-Gal4 (#2318), Gr5a-GAL4 (#57592), and UAS-GCaMP6m (#42748) were obtained from the Bloomington Drosophila Stock Center at Indiana University; UAS-E(z) RNAi (#2831), UAS-Pcl RNAi (#1185), UAS-Su(var)3–9 RNAi (#3558), UAS-Rpd3 RNAi (#0695), and UAS-cad RNAi (#03,877.N) were from the Tsinghua Fly Center.

Flies (Drosophila melanogaster) were reared on a standard corn meal, yeast and glucose agar medium at 24.5 °C under a 12-h:12-h light: dark (LD) cycle as described before unless otherwise stated^{10 (link)}. Control (w¹¹¹⁸) and UAS-TrpA1 fly stocks were described previously^{10 (link),13 (link)}. Gr5a-GAL4 (#57592), Gr43a-GAL4 (#57636) and Gr64a-GAL4 (#57661) were obtained from the Bloomington Stock Center; Cha-GAL80, Gad-GAL80 and UAS-Shi^ts1 were from Dr. T. Sakai (Tokyo Metropolitan University). To remove possible modifiers and allow for comparisons in a common genetic background, we outcrossed all the strains into a control (w¹¹¹⁸) background for at least five consecutive generations.