LN-18 and A172 cells were lysed with a Pierce™ IP Lysis Buffer (Thermo Fisher Scientific). Equal amount of extracted protein samples were then blotted onto a nitrocellulose membrane (Shanghai Genepharma). A WT1 polyclonal antibody (1:500, Invitrogen) and a polyclonal beta-actin antibody (1:10,000 Invitrogen) were used for primary Western blot reaction, and horseradish peroxidase-conjugated secondary antibodies (Invitrogen) were used for secondary Western blot reaction. Finally, the blots were visualized using a Fusion Solo chemiluminescence system (PEQLAB Biotechnologie GmbH, Germany) according to the manufacturer’s recommendation.
Beta actin antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Beta-actin antibody is a laboratory reagent used to detect and quantify the beta-actin protein, a ubiquitous and highly-conserved cytoskeletal protein. It is commonly used as a loading control or reference standard in various biological assays, such as Western blotting and immunohistochemistry, to ensure equal sample loading or to normalize protein expression data.
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Lab products found in correlation
2 protocols using beta actin antibody
1
Western Blot Analysis of WT1 Expression
2
Investigating EGFR Phosphorylation in OVCAR-3 Cells
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In the study, OVCAR-3 cells were seeded into 75 cm2 (link) culture flasks and incubated until the logarithmic phase was achieved. When the cells reached the logarithmic phase, vehicle control, DOX IC50 = 0.08 μM, and RA IC50 = 437.6 μM were administered singly and in combination. Protein isolation was done from the samples 72 hours after the application. In the study, Western Breze brand ready kits provided by Thermo Scientific firm were used, blotting and transfer to the membrane were carried out using the iBlot 2 (Life Technologies) system, ready-made membranes and kit, following the kit protocols. After blotting, the proteins were treated with Anti-EGFR (Phospho-Tyr1197) Antibody (ABM, CAT no: Y011228), Beta actin Antibody (Invitrogen, CAT no: MA1-140) specific primary antibodies, then antibodies were labeled with appropriate secondary and Micro Observed with the ChemiDoc (DNR Bio-Imaging Systems Ltd, United States of America) gel imaging system. Band intensities were calculated using GelQuant software.
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