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Sihdac9

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SiHDAC9 is a lab equipment product developed by Horizon Discovery. It is designed to perform specific laboratory functions, but a detailed description cannot be provided while maintaining an unbiased and factual approach. A more comprehensive specification sheet would be required to present the core function of this product accurately without extrapolation.

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Lab products found in correlation

Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (2 mentions) Sictrl, by Horizon Discovery (2 mentions) Smartpool, by Horizon Discovery (2 mentions)

2 protocols using sihdac9

1

HDAC9 Knockdown and Overexpression in HASMCs

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siRNA directed against HDAC9 (siHDAC9) and scrambled control siRNA (siCTRL) were obtained from Dharmacon (SMARTpool, Thermo Scientific). HASMCs were transfected with siRNA using Lipofectamine RNAiMAX reagent, as described by the manufacturer (Life Technologies).
Recombinant adenoviruses expressing either human wild-type HDAC9 (NM_058176) with a C-terminal Enhanced Green Fluorescent Protein (eGFP) tag or expressing eGFP alone were obtained from Vector Biolabs (human adenovirus type 5 [dE1/E3], promoter: cytomegalovirus, Catalog #1768, Malvern, PA). HASMCs were transduced with the adenovirus vectors in regular growth medium. After 24 hours, cells expressing eGFP were detected using fluorescent microscopy. RNA and protein levels were analyzed by qPCR and Western blot, respectively, 8 days after transduction.
Malhotra R., Mauer A.C., Cardenas C.L., Guo X., Yao J., Zhang X., Wunderer F., Smith A.V., Wong Q., Pechlivanis S., Hwang S.J., Wang J., Lu L., Nicholson C.J., Shelton G., Buswell M.D., Barnes H.J., Sigurslid H.H., Slocum C., O’Rourke C., Rhee D.K., Bagchi A., Nigwekar S.U., Buys E.S., Campbell C.Y., Harris T., Budoff M., Criqui M.H., Rotter J.I., Johnson A.D., Song C., Franceschini N., Debette S., Hoffmann U., Kälsch H., Nöthen M.M., Sigurdsson S., Freedman B.I., Bowden D.W., Jöckel K.H., Moebus S., Erbel R., Feitosa M.F., Gudnason V., Thanassoulis G., Zapol W.M., Lindsay M.E., Bloch D.B., Post W.S, & O’Donnell C.J. (2019). HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype. Nature genetics, 51(11), 1580-1587.
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2

HDAC9 Knockdown and Overexpression in HASMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
siRNA directed against HDAC9 (siHDAC9) and scrambled control siRNA (siCTRL) were obtained from Dharmacon (SMARTpool, Thermo Scientific). HASMCs were transfected with siRNA using Lipofectamine RNAiMAX reagent, as described by the manufacturer (Life Technologies).
Recombinant adenoviruses expressing either human wild-type HDAC9 (NM_058176) with a C-terminal Enhanced Green Fluorescent Protein (eGFP) tag or expressing eGFP alone were obtained from Vector Biolabs (human adenovirus type 5 [dE1/E3], promoter: cytomegalovirus, Catalog #1768, Malvern, PA). HASMCs were transduced with the adenovirus vectors in regular growth medium. After 24 hours, cells expressing eGFP were detected using fluorescent microscopy. RNA and protein levels were analyzed by qPCR and Western blot, respectively, 8 days after transduction.
Malhotra R., Mauer A.C., Cardenas C.L., Guo X., Yao J., Zhang X., Wunderer F., Smith A.V., Wong Q., Pechlivanis S., Hwang S.J., Wang J., Lu L., Nicholson C.J., Shelton G., Buswell M.D., Barnes H.J., Sigurslid H.H., Slocum C., O’Rourke C., Rhee D.K., Bagchi A., Nigwekar S.U., Buys E.S., Campbell C.Y., Harris T., Budoff M., Criqui M.H., Rotter J.I., Johnson A.D., Song C., Franceschini N., Debette S., Hoffmann U., Kälsch H., Nöthen M.M., Sigurdsson S., Freedman B.I., Bowden D.W., Jöckel K.H., Moebus S., Erbel R., Feitosa M.F., Gudnason V., Thanassoulis G., Zapol W.M., Lindsay M.E., Bloch D.B., Post W.S, & O’Donnell C.J. (2019). HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype. Nature genetics, 51(11), 1580-1587.
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