The levels of IL-5 and IL-13 in BAL fluid were measured by Quantikine and Duoset ELISA kits (R&D Systems) according to the manufacturer’s instructions. Total IgE was determined by the Clonotyping ELISA kits (Southern Biotech). Ovalbumin-specific IgE in mouse sera was quantified as following. Immulon 2HB plates were coated with 2 μg ovalbumin in bicarbonate buffer (pH 9.6) per well overnight at 4 °C and blocked with 1% BSA. Serum samples were diluted in 0.1% Tween 20-PBS and incubated for 2 hours at room temperature. OVA-specific-IgE was detected by using goat-anti-mouse IgE conjugated with HRP (Southern Biotech). Plates were developed with TMB substrate solution (R&D Systems) and reactions were stopped with 1N HCl. Absorbance values at OD450 were measured. Concentrations of ovalbumin-specific IgE were determined using a standard curve made with serum from hyper-immunized mice.
Clonotyping elisa kits
Manufactured by Southern Biotech
Clonotyping ELISA kits are laboratory equipment used to detect and quantify specific T-cell receptor sequences in a sample. They provide a standardized method for analyzing the diversity and frequency of T-cell clones within a population.
Automatically generated - may contain errors
2 protocols using clonotyping elisa kits
1
Quantification of Allergy Biomarkers in BAL Fluid
2
Autoantibody Profiling in Mice
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Anti-parietal cell antibodies and RF titers were assessed by ELISA as previously described (Sakaguchi et al., 2003 (link); Takahashi et al., 1998 (link)). Anti-CCP antibody was assessed by MESACUP-2 CCP kit using anti-mouse Ig antibody (MBL). Serum IgG, IgG1, and IgG2a levels were measured by ELISA using either Mouse IgG ELISA Quantitation Kit (Bethyl Laboratories) or Clonotyping Elisa kits (Southern Biotech). IgE levels were measured by OptEIA Mouse IgE ELISA set (BD Biosciences).
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