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Pan histone h3

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Pan histone H3 is a lab equipment product that detects histone H3, a core histone protein found in eukaryotic cell nuclei. It is used for the identification and analysis of histone H3 in various research applications.

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Lab products found in correlation

Hdac7, by Cell Signaling Technology (2 mentions) Hnrnpl 4d11, by Abcam (2 mentions) Flag tag (flag), by Cell Signaling Technology (2 mentions) Lamin b1, by Abcam (2 mentions) H3k27ac, by Abcam (2 mentions) H3k9ac, by Abcam (2 mentions)

2 protocols using pan histone h3

1

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For protein analysis, 5–30 μg of whole cell protein extract (WCE) were loaded into 6%–15% 37.5:1 bis-acrylamide SDS-PAGE gels, depending on protein of interest size. Antibodies for Western blot were diluted in 5% BSA-TBST as follows: HDAC7 (Cell Signaling D4E1L, 1:1000), Lamin-B1 (Abcam ab133741; 1:5000), PKM2 (Cell Signaling D78A4; 1:1000), Flag (Cell Signaling, 1:1000-2000), pan histone H3 (Abcam ab1791), H3K9ac (Abcam ab4441), H3K27ac (Abcam ab4729), HNRNPL (4D11, Abcam ab6106).
Agosto L.M., Mallory M.J., Ferretti M.B., Blake D., Krick K.S., Gazzara M.R., Garcia B.A, & Lynch K.W. (2023). Alternative splicing of HDAC7 regulates its interaction with 14-3-3 proteins to alter histone marks and target gene expression. Cell reports, 42(3), 112273.
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2

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For protein analysis, 5–30 μg of whole cell protein extract (WCE) were loaded into 6%–15% 37.5:1 bis-acrylamide SDS-PAGE gels, depending on protein of interest size. Antibodies for Western blot were diluted in 5% BSA-TBST as follows: HDAC7 (Cell Signaling D4E1L, 1:1000), Lamin-B1 (Abcam ab133741; 1:5000), PKM2 (Cell Signaling D78A4; 1:1000), Flag (Cell Signaling, 1:1000-2000), pan histone H3 (Abcam ab1791), H3K9ac (Abcam ab4441), H3K27ac (Abcam ab4729), HNRNPL (4D11, Abcam ab6106).
Agosto L.M., Mallory M.J., Ferretti M.B., Blake D., Krick K.S., Gazzara M.R., Garcia B.A, & Lynch K.W. (2023). Alternative splicing of HDAC7 regulates its interaction with 14-3-3 proteins to alter histone marks and target gene expression. Cell reports, 42(3), 112273.
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