Following decalcification, 7 µm thick paraffin-embedded tissue samples were prepared and subjected to hematoxylin-eosin (H&E) and Alcian blue staining. Sections were then imaged and measured with an Olympus BX51 microscope and a DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo).
Dp73 ccd imaging system
Manufactured by Olympus
Sourced in Japan
The DP73 CCD Olympus Imaging System is a high-performance digital camera designed for microscopy applications. It features a 12.8-megapixel CCD sensor and provides fast, high-resolution image capture capabilities. The system is equipped with advanced image processing and analysis functions to support a wide range of research and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Bx51 microscope, by Olympus (4 mentions)
Bcip nbt alkaline phosphatase color development kit, by Beyotime (2 mentions)
G1202, by Wuhan Servicebio Technology (1 mentions)
G5001, by Wuhan Servicebio Technology (1 mentions)
Bovine serum albumin (bsa), by Wuhan Servicebio Technology (1 mentions)
G1105 500ml, by Wuhan Servicebio Technology (1 mentions)
Caseviewer 2, by 3DHISTECH (1 mentions)
8 protocols using dp73 ccd imaging system
1
Tissue Histomorphological Analysis
2
Histological Analysis of Callus Formation
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The reserved callus tissues were fixed overnight in 4% paraformaldehyde. Sections of paraffin-embedded callus (5 μm thick) were then stained with hematoxylin, eosin (H&E), alcian blue, and proliferating nuclear antigen (PCNA). For immunohistochemistry, the callus sections were dewaxed, and rehydrated, followed by antigen retrieval using ethylene diamine tetraacetic acid-citrate buffer (pH = 6.0; G1202, Servicebio, Wuhan, Hubei, China). The sections were blocked with bovine serum albumin (BSA) (G5001, Servicebio) and then incubated overnight at 4°C with the primary antibody to PCNA (ab29, Abcam, Cambridge, MA, USA). After incubation with secondary goat anti-mouse immunoglobulin G (IgG) for 50 min, the sections were fixed and then imaged using DP73 CCD Olympus imaging system (Olympus Corp., Tokyo, Japan) and Olympus BX51 microscope.
3
Decalcification and Histological Evaluation
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After micro-CT scanning, the samples were decalcified in 10% EDTA for 2 months, during which time the 10% EDTA solution was changed every week. The samples were embedded in paraffin and sectioned at a thickness of 5 μm for H&E staining. The H&E staining slides were observed under an optical microscope, and the images were captured under the Olympus microscope BX51 and DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo, Japan). Histological evaluation was performed by two independent examiners.
4
Immunohistochemical Analysis of FOS Expression in Ovarian Tissue
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The ovarian tissue sections were dewaxed and rehydrated, followed by treatment with antigen recovery solution (pH 6.0 EDTA citrate buffer; Servicebio, G1202, China), and sealing with BSA (Servicebio, G5001, China). The sections then underwent incubation overnight with primary antibody (anti‐FOS, ab190289, Abcam, Cambridge, UK) at 4℃. After incubation with the secondary Goat anti‐rabbit IgG for 50 min, the sections were fixed. The sections were then imaged with Olympus BX51 microscope and DP73 CCD Olympus imaging system (Olympus Corporation, Tokyo, Japan).
5
Femoral Histomorphometry via Decalcification and Staining
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After completion
of the CT scan, the femurs were placed in a decalcification fluid
(Servicebio, G1105-500ML, Wuhan, China) for three consecutive weeks,
with the decalcification fluid being changed every 3 days. The decalcified
femur was embedded with paraffin and sectioned in the direction of
the long axis of the femoral stem with a thickness of 5 μm.
The sections were fixed on the slides and stained with H&E and
Alcian blue. The sections were imaged with an Olympus BX51 microscope
and a DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo).
Caseviewer 2.4 (3DHISTECH Ltd, Hungary) was used to observe and analyze
the morphology of the specimens at 0.8×, 5×, and 20×
magnifications. ImageJ was used to calculate the area of bone and
cartilage of the callus.
of the CT scan, the femurs were placed in a decalcification fluid
(Servicebio, G1105-500ML, Wuhan, China) for three consecutive weeks,
with the decalcification fluid being changed every 3 days. The decalcified
femur was embedded with paraffin and sectioned in the direction of
the long axis of the femoral stem with a thickness of 5 μm.
The sections were fixed on the slides and stained with H&E and
Alcian blue. The sections were imaged with an Olympus BX51 microscope
and a DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo).
Caseviewer 2.4 (3DHISTECH Ltd, Hungary) was used to observe and analyze
the morphology of the specimens at 0.8×, 5×, and 20×
magnifications. ImageJ was used to calculate the area of bone and
cartilage of the callus.
6
Histological Tissue Analysis Protocol
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Following decalcification, 5–7 µm thick paraffin-embedded tissue samples were prepared and subjected to H&E and Alcian blue staining. Sections were then imaged and measured with an Olympus BX51 microscope and a DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo).
7
Alkaline Phosphatase Colorimetric Assay for Cell Imaging
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A BCIP/NBT alkaline phosphatase color development kit (#C3206, Beyotime, China) was utilized based upon provided directions. Brie y, cells were washed twice by using PBS, after wich 10% formalin was added to x the cells for 15 minutes. BCIP/NBT substrate was then used to treat cells for 24 h, and colorimetric changes were analyzed using a charge-coupled microscope, with a scanner used to image stained cells. Absorbance was then measured at 405 nm. Experiments were repeated in triplicate.
Mice fracture model and treatment microscope and a DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo).
Mice fracture model and treatment microscope and a DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo).
8
Alkaline Phosphatase Colorimetric Assay for Cell Imaging
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A BCIP/NBT alkaline phosphatase color development kit (#C3206, Beyotime, China) was utilized based upon provided directions. Brie y, cells were washed twice by using PBS, after wich 10% formalin was added to x the cells for 15 minutes. BCIP/NBT substrate was then used to treat cells for 24 h, and colorimetric changes were analyzed using a charge-coupled microscope, with a scanner used to image stained cells. Absorbance was then measured at 405 nm. Experiments were repeated in triplicate.
Mice fracture model and treatment microscope and a DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo).
Mice fracture model and treatment microscope and a DP73 CCD Olympus Imaging System (Olympus Corporation, Tokyo).
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