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Zinc 2 sulfate heptahydrate

Manufactured by Merck Group
Sourced in Australia, India, Spain

Zinc (II) sulfate heptahydrate is a chemical compound with the formula ZnSO4·7H2O. It is a colorless, crystalline solid that is soluble in water. The primary function of zinc (II) sulfate heptahydrate is as a source of zinc ions in various laboratory applications and experiments.

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4 protocols using zinc 2 sulfate heptahydrate

1

Trace Metal Analysis in Anaerobic Media

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The amount of metal in fresh media (Anaerobic Basal Broth) was analyzed via Inductively Coupled Plasma Mass Spectroscopy by the Wisconsin State Lab of Hygiene.
In metal supplementation experiments, supernatants were supplemented with iron (II) sulfate (Alfa Aesar), manganese (II) sulfate monohydrate (Sigma-Aldrich), nickel sulfate (II) hexahydrate (Sigma-Aldrich), zinc (II) sulfate heptahydrate (Alfa Aesar), and/or cobalt (II) chloride hexahydrate (Sigma-Aldrich). Metal compounds were prepared as 100× stocks in water and filter sterilized with 0.2 μM filters.
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2

Purification of Histidine-Tagged Proteins

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Tryptone and yeast extract were purchased from Bacto (NSW, Australia). Complete, EDTA-free protease inhibitor tablets were obtained from Roche (Sigma-Aldrich, NSW, Australia). Deoxyribonuclease I from bovine pancreas was purchased from Sigma-Aldrich (NSW, Australia). n-Dodecyl-β-d-maltopyranoside (DDM) was obtained from Anatrace (Ohio, USA). Ni Sepharose high-performance histidine-tagged protein purification resin (HP resin) was purchased from GE Healthcare (NSW, Australia). Total E. coli lipid extract and egg l-α-phosphatidylcholine (egg PC) were obtained from Avanti Polar Lipids (Alabama, USA). Transition metal ion salts manganese(II) sulfate monohydrate, zinc(II) sulfate heptahydrate, copper(II) sulfate heptahydrate, cobalt(II) chloride, and nickel(II) chloride were purchased from Sigma-Aldrich (NSW, Australia). The Transcreener ADP2 FP Assay Kit was obtained from Bellbrook Labs (Wisconsin, USA).
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3

Synthesis of Metal-Organic Complexes

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Citric
acid, mercury(II)
acetate, cobalt(II) chloride hexahydrate, nickel(II) chloride hexahydrate,
cadmium(II) chloride, copper(II) chloride dihydrate, iron(II) sulfate
heptahydrate, manganese(II) acetate tetrahydrate, lead nitrate, zinc(II)
sulfate heptahydrate, sodium chloride, and sodium hydroxide were purchased
from Merck, India. p-Phenylenediamine was purchased
from Loba Chemie, and terbium(III) chloride and dialysis bags (1 kDa)
were purchased from Sigma-Aldrich. All reagents were of analytical
grade and used without further purification. Milli-Q water was used
for all the experiments.
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4

Antioxidant Potential of Metal Salts

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Potassium peroxymonosulfate (PMS) was acquired from Alfa Aesar (Ward Hill, Massachusetts, US), aluminum sulfate 18-hydrate (10% w/w, Al2(SO4)3•18H2O) was obtained from Scharlau (Barcelona, Spain), cobalt(II) sulfate heptahydrate, iron(II) sulfate heptahydrate (FeSO4•7H2O) and manganese(II) sulfate monohydrate (MnSO4•H2O) were acquired from Panreac (Barcelona, Spain), Zinc(II) sulfate heptahydrate was acquired from Merck, (Darmstadt, Germany), copper(II) sulfate 2-hydrate (CuSO4•2H2O) was acquired from Honeywell Riedel-de-Haën™ (Charlotte, North Caroline, USA), magnesium sulfate heptahydrate (MgSO4•7H2O), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), catechin, gallic acid, caffeic acid and sodium chloride (NaCl) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For pH adjustment, sodium hydroxide (NaOH) was used from Labkem (Barcelona, Spain) and sulfuric acid (H2SO4, 95%) from Scharlau (Barcelona, Spain). Deionized water was used to prepare the respective solutions.
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