To form xenografts, 4 million PC3 cells in 1:1 DMEM:matrigel were injected into the left flank of female nude mice (Charles River Laboratories). Tumor volume was measured with calipers and calculated by using the formula V = (1/2) (L*W2), where L and W are length and width, respectively. To assess glucose metabolism in the tumors, uniformly 13C-labeled ([U-13C6]) glucose was introduced via intratumoral infusion. A standard solution of [U-13C6] glucose was first prepared by suspending 200 mg [U-13C6] glucose into 1 mL of 0.9% saline. The amount of [U-13C6] glucose solution injected into each mouse tumor was normalized by individual tumor volumes (40 μL of stock [U-13C6] glucose per 200 mm3 tumor volume). One hour after [U-13C6] glucose labeling, tumors were collected and stored at −80°C until extraction for LC/MS analysis.
Female nude mice
Manufactured by Charles River Laboratories
Sourced in China, United States, Japan
Female nude mice are laboratory animals that lack fur due to a genetic mutation. They are commonly used in medical research and drug development to study various diseases and test new treatments.
Automatically generated - may contain errors
Lab products found in correlation
Matrigel, by BD (5 mentions)
Matrigel, by Corning (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Matrigel basement membrane matrix, by BD (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Bevacizumab, by Genentech (2 mentions)
Matrigel basement membrane matrix, by Merck Group (2 mentions)
Ab137785, by Abcam (2 mentions)
Za 0502, by ZSGB-BIO (2 mentions)
Female balb c mice, by Charles River Laboratories (2 mentions)
Doxycycline hyclate, by Merck Group (1 mentions)
Ivis100 imager, by PerkinElmer (1 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions)
Female balb c mice, by Jackson ImmunoResearch (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Ht 29, by American Type Culture Collection (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
C57bl 6 male mice, by Jackson ImmunoResearch (1 mentions)
100 protocols using female nude mice
1
Xenograft Glucose Metabolism Labeling
2
Colon Cancer Xenograft Model in Nude Mice
3
Evaluating Combinatorial Cancer Therapy
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The animal experiments were approved by the Committee on the Ethics of Animal Experiments of Chiba Cancer Center (Permission Number: 18‐1) and carried out in compliance with the institutional guidelines for the care and use of animal research and the ARRIVE Guidelines 2.0.28 A549 cells (2 × 106) were injected subcutaneously into 4‐week‐old female nude mice (Charles River Laboratories Japan). When the average tumor size reached approximately 80 mm3, the mice were randomized into four groups. Mice in the control group (n = 5) received vehicle alone (2% DMSO/30% PEG300/2% Tween‐80/PBS). Mice in the CCC‐021‐TPP group (n = 5) and the A‐1155463 group (n = 5) were intraperitoneally treated with CCC‐021‐TPP (5 mg/kg body weight) twice a week and A‐1155463 (5 mg/kg body weight) daily, respectively. Mice in the combination group (n = 5) were injected with CCC‐021‐TPP and A‐1155463 twice a week and daily, respectively. Tumor volume was estimated from the equation of a × b2/2, where a and b represent long and short diameter, respectively. On day 21, the mice were killed by carbon dioxide for approximately 15 minutes after respiratory arrest to ensure minimal suffering. Tumor tissues were removed and subjected to histochemical analyses.
4
Nude Mice Xenograft Model for Erufosine Treatment
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Female nude mice were obtained (Charles River, Germany) at an age of 4 to 6 weeks and kept under specific pathogen free (SPF) conditions in Macrolon-III-cages of a ventilated rack. Constant room temperature (22 ± 1°C), air humidity (50 ± 10%) and dark-light-rhythm (12 h) were maintained throughout. The animals had free access to autoclaved water and standard laboratory diet. After an adaptation period of a week, experiments were started. All animal experiments were performed in accordance with institutional and governmental regulations, and were approved by the Regierungspräsidium (Karlsruhe, Germany).
HN-5 cells (1.5 × 106/100 µl) were injected subcutaneously into the flanks of the animals, with 6 animals being used in each experimental group. After 4–5 days of tumor development, mice received either PBS or erufosine as treatment, administered intraperitoneally twice a week (60 µmole/kg or 30 mg/kg). Animal weight and tumor dimensions were measured weekly and tumor volume was calculated as a × b2 × 0.5, where a > b. After 4 weeks, animals were sacrificed and tumors were isolated for western blotting and IHC staining’s. The experiments were performed in duplicates.
HN-5 cells (1.5 × 106/100 µl) were injected subcutaneously into the flanks of the animals, with 6 animals being used in each experimental group. After 4–5 days of tumor development, mice received either PBS or erufosine as treatment, administered intraperitoneally twice a week (60 µmole/kg or 30 mg/kg). Animal weight and tumor dimensions were measured weekly and tumor volume was calculated as a × b2 × 0.5, where a > b. After 4 weeks, animals were sacrificed and tumors were isolated for western blotting and IHC staining’s. The experiments were performed in duplicates.
5
Inducible TUSC2 Overexpression in Murine Glioma
6
Combination Therapy for MCF-7 Xenograft Treatment
7
Xenograft Tumor Model Development
8
Xenograft Breast Cancer Tumor Regression
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Female nude mice
(4–6 weeks old) were purchased from Charles River. All in vivo
experiments were carried out under the supervision of the Institutional
Animal Care and Use Committee (IACUC) of Virginia Tech. The tumor
shrinkage efficacy of Ch-MLNPs was evaluated in a xenograft breast
cancer model. In brief, 5 × 107 cells/mL MDA-MB-231
cells (0.1 mL) (50:50 mixed with BD Matrigel Basement Membrane Matrix)
were injected subcutaneously into the hind flanks of 8-week-old BALB/c
nude mice. Tumors were allowed to form for 2–3 weeks. When
the tumor reached a volume of 100 mm3, NP treatments were
performed by intravenously administering NPs at the MTD via tail vein.
Perpendicular tumor diameters were measured by digital caliper and
used to calculate tumor volume according to the reported protocol
every two days.70 (link),71 (link) Mice were sacrificed when the
tumors reached a volume of 600 mm3.
(4–6 weeks old) were purchased from Charles River. All in vivo
experiments were carried out under the supervision of the Institutional
Animal Care and Use Committee (IACUC) of Virginia Tech. The tumor
shrinkage efficacy of Ch-MLNPs was evaluated in a xenograft breast
cancer model. In brief, 5 × 107 cells/mL MDA-MB-231
cells (0.1 mL) (50:50 mixed with BD Matrigel Basement Membrane Matrix)
were injected subcutaneously into the hind flanks of 8-week-old BALB/c
nude mice. Tumors were allowed to form for 2–3 weeks. When
the tumor reached a volume of 100 mm3, NP treatments were
performed by intravenously administering NPs at the MTD via tail vein.
Perpendicular tumor diameters were measured by digital caliper and
used to calculate tumor volume according to the reported protocol
every two days.70 (link),71 (link) Mice were sacrificed when the
tumors reached a volume of 600 mm3.
9
In Vivo Photothermal Therapy Protocol
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Animal experiments were carried out with approval from the University at Buffalo Institutional Animal Care and Use Committee. Panc-1 tumours were grown by injecting 6-week-old female nude mice (Charles River) with 3 × 106 Panc-1 cells in a 1:1 Matrigel dilution (BD Biosciences) in the hind flank of the mice. Following several weeks of growth, the tumours were carefully injected intratumorally with sulforhodamine B–PoP-liposomes. Images were acquired with an in vivo fluorescence imager (IVIS Lumina II) at the indicated time points with the mice under isoflurane-induced anaesthesia. The tumour was irradiated for 30 min with a 0.6-cm diameter spot size at 200 mW cm−2 power density and mice were imaged again.
10
Xenograft Model of Breast Cancer
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The MDA-MB-231-Luc-D3H2LN cell line was purchased from Xenogen Corporation. After infection with the appropriate recombinant lentivirus, these cells were inoculated into the left abdominal mammary fat pad (3 × 106 cells) of 8-week-old female nude mice (Charles River, Beijing, China), each group including eight mice, and the animals were allocated to experimental groups using method of randomization. The details of the bioluminescence imaging procedure were as described earlier48 (link). The animal procedures were approved by the Peking University Health Science Center Institutional Animal Care and Use Committee.
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