Isopropyl alcohol ipa

The AuNPs were purchased from Sigma Aldrich (St. Louis, MO, USA) in 25 mL bottles in 0.1 mM PBS. The studied particle diameters were 20 nm (product no 753610), 40 nm (product no 753637), 60 nm (product no 753653), 80 nm (product no 753661), and 100 nm (product no 753688). Methanol (34885-1L), Acetone (product number 179124), Isopropyl alcohol (IPA) (product number W292907), and Acetonitrile (product number 271004) were also purchased from Sigma Aldrich. Quartz cuvettes (product name CV10Q700F) for UV-VIS absorbance measurements were purchased from Thorlabs. The experimental UV-VIS data were collected using an Ocean Insight (Geograaf, EW Duiven, The Netherlands) HR2000+ spectrometer (product name HR2000CG, Ocean Insight). An Ocean Insight CUV-ALL-UV cuvette holder (product name CUV-UV, Ocean Insight) was used. The light source used was a DH-2000 BAL deuterium halogen light source (product number DH-2000-BAL, Ocean Insight).

Urethane dimethacrylate (UDMA), triethylene glycol dimethacrylate (TEGDMA), diethylene glycol dimethacrylate (DEGDMA) and ethylene glycol dimethacrylate (EGDMA) were purchased from Sigma Aldrich (Gillingham, UK) (Fig. 1). Diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide (TPO) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Isopropyl alcohol (IPA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). NIH 3T3 embryonic mouse fibroblasts (ATCC-CRL-1658), Dulbecco’s modified Eagle’s medium (DMEM) and calf bovine serum (CBS) were purchased from ATCC (LGC, UK).Fig. 1

Chemical structures of Urethane dimethacrylate, diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide, ethylene glycol dimethacrylate, diethylene glycol dimethacrylate and triethylene glycol dimethacrylate

Silver nitrate (AgNO₃, 169.87 g/mol; 99.99% purity) and DDoAC (586.50 g/mol; ≥97%) were purchased from Sigma–Aldrich (Milan, Italy). DDAC (362.08 g/mol; ≥95%) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Milli-Q water and isopropyl alcohol (IPA, anhydrous, 99.5%, Sigma Aldrich, Milan, Italy) were used throughout the experiments.

All chemicals were the highest purity available and were used as received without further purification or distillation. 1,2-Dipalmitoyl-sn-glycero-3-phosphocoline (DPPC, >98%), Cholesterol (>99%, stabilized with α-Tocopherol) were purchased from Tokyo Chemical Industry UK Ltd (Belgium). 16:0 PEG2000 PE 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (ammonium salt) was purchased from AVANTI^® Polar Lipids, INC (Molecular structures can be seen in Figure 1). Lysozyme (from chicken egg white, lyophilized powder, protein $\ge$ 90%, $\ge$ 40,000 units/mg protein), tablets of phosphate-buffered saline (PBS, pH 7.4), ethanol, isopropyl alcohol (IPA), and acetonitrile $\ge$ 99.8% were obtained by Sigma-Aldrich (Steinheim, Germany). Microfluidic devices were fabricated using an Asiga MAX UV 3DP and PlasCLEAR resin (Asiga, Alexandria, Australia).

Lidocaine USP and prilocaine USP were purchased from Spectrum Chemical Mfg. Corp. (Gymea, NSW, Australia). Phosphate-buffered saline (PBS) sachets (pH 7.4), isopropyl alcohol (IPA), sodium azide, isopropyl myristate (IPM), sodium lauryl sulfate (SLS), acetonitrile, sodium hydroxide, ethanol, di-sodium hydrogen phosphate, and sodium phosphate monobasic of analytical grade were purchased from Sigma-Aldrich (Macquarie Park, NSW, Australia). Carbopol 980 and Volpo™ N20 were prepared by Lubrizol Corporation (Silverwater, NSW, Australia) and Croda (Parramatta, NSW, Australia), respectively. Milli-Q water was used in this work, unless otherwise noted.