All experimental procedures were approved by the Sungkyunkwan University Institutional Animal Care and Use Committee and were conducted in accordance with the Guide for the Care and Use of Laboratory Animals of the Animal Protection Law & the Laboratory Animal Act set by the Korea Animal and Plant Quarantine Agency and the Korea Ministry of Food and Drug Safety. We used adult male C57BL/6 mice (n = 20; Orient Bio, South Korea), male Thy1-GCaMP6f mice (n = 10, C57BL/6J-TgGP5.17DKim/J, stock no.025939, Jackson Laboratory, USA) and adult male C57BL/6 mice (n = 10; Orient Bio) with viral expression of GCaMP6f (AA9-mDlx-GCaMP6f-Fishell-2, plasmid#83899, Addgene, USA). All mice were maintained under a 12-h dark/light cycle, 24–25°C temperature and 50–60% humidity. Experiments were carried out on 10- to 14-week old mice.
C57bl 6 mice
Manufactured by Orient Bio
Sourced in United States, Cameroon
C57BL/6 mice are a widely used inbred mouse strain. They are a commonly employed model organism in biomedical research due to their well-characterized genetic background.
Automatically generated - may contain errors
Lab products found in correlation
Balb c, by Orient Bio (8 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Xylazine, by Bayer (5 mentions)
Rompun, by Bayer (5 mentions)
Lipopolysaccharides (lps), by Merck Group (4 mentions)
Sprague dawley rats, by Orient Bio (4 mentions)
C57bl 6, by Orient Bio (3 mentions)
Balb c nude mice, by Orient Bio (3 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Vaseline lanette cream, by Fagron (2 mentions)
Wistar rats, by Orient Bio (2 mentions)
2 mercaptoethanol, by Merck Group (2 mentions)
Ficoll, by Merck Group (2 mentions)
Liberase, by Roche (2 mentions)
Methylcellulose, by Merck Group (2 mentions)
B6129sf2 j, by Jackson ImmunoResearch (2 mentions)
442 protocols using c57bl 6 mice
1
Calcium Imaging in Transgenic Mice
2
C57BL/6 Mouse Husbandry and Welfare
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Adult male C57BL/6 mice (7 weeks old) were purchased from Orient Bio, Inc. (Seongnam, South Korea), a branch of the Charles River Laboratories. The mice were maintained at 21 °C under a 12-h light: dark cycle and had ad libitum access to water and rodent chow. Every effort was made to minimize animal suffering. All experiments were performed in accordance with the National Institutes of Health and Ewha Womans University guidelines for laboratory animal care and use, and the study was approved by the Institutional Animal Care and Use Committee of the Medical School of Ewha Womans University (#EUM 20-022). The study was carried out in compliance with the ARRIVE guidelines.
3
Assessing Neurological Function in Aging Mice
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Animal experiments were conducted in accordance with approved guidelines of Seoul National University Institutional Animal Care and Use Committee (IACUC No. SNU-180430-6). Male C57BL/6 mice (Orientbio, Sungnam, Republic of Korea) were housed in the animal facility of Seoul National University. Young (12-week-old) and aged (older than 24-month-old) mice were used for experiments. After intravenous application of hUCP, neurological function of mice was assessed. The mice did not represent immune rejection-related signs, including sudden death, weight loss and inflammation, caused by a xenotransplantation. After the mice were anesthetized, brain, spleen and blood samples were collected for more ex vivo examinations.
4
Laser-Induced Retinal Nerve Damage in Mice
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Example 7
C57BL/6 mice were purchased from Orient Bio and the animal experiments was carried out in accordance with the guidelines approved by the University of Inje (No, 2013-053) and ARVO regarding animal use for eye and vision studies. 6-week-old C57BL/6 mice were treated with diode green laser (532 nm, 150 mW, 0.1 sec, 50 μM, photocoagulator) to damage the retinal optic nerve area. Immediately after laser irradiation, CP1, CPII and positive control group Avastin were dissolved in PBS and administered 5 mg each per a day in the eyeball for 5 days. The above experiment for each experiment group was performed using both eyes of five mice each.
5
Mouse Model for Metabolic Studies
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Adult male C57BL/6 mice (9 weeks of age) were purchased from Orient Bio Inc. (Seongnam, Korea), a branch of Charles River Laboratories. Mice were maintained at 21°C under a 12 h light:12 h dark cycle and had ad libitum access to water and rodent chow. Every effort was made to minimize animal suffering. All experiments were performed in accordance with the National Institutes of Health (NIH, Bethesda, MD, USA) and Ewha Womans University (Seoul, Korea) guidelines for laboratory animal care and use, and the study was approved by the Institutional Animal Care and Use Committee of the Medical School of Ewha Womans University (#EUM 20-022).
6
Prophylactic Chemical Treatments for Psoriasis-like Skin Inflammation in Mice
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All mouse experimental procedures were approved by the Animal Care and Use Committee of the Ewha Womans University School of Medicine (ESM-17-0388). C57BL/6 mice (male, 18-20 g, 8 weeks of age) were purchased from OrientBio (Seongnam, South Korea) and housed under specific-pathogen-free conditions on a 12 h light:12 h dark cycle with free access to food and water. To induce psoriasis-like skin inflammation, mice were treated once daily with a topical dose of 83 mg (18) of IMQ cream applied to the shaved back region for 6 consecutive days, as described previously (19) . Normal control mice were treated similarly with a control vehicle cream (Vaseline Lanette cream, Fagron, Rotterdam, The Netherlands). For prophylactic chemical treatment, mice were exposed once daily to topically administered MP-A08, Ceranib-2, PF-543, or ABC294640. MP-A08, Ceranib-2, and ABC294640 were used at 25 µg/40 µl surfactant (100% ethanol, propylene glycol, and H 2 O (EPH) at 2:1:1 (v/v/v)); PF-543 was used at 12.5 µg/40 µl surfactant applied to the shaved back skin. Separate control groups received the vehicle control (EPH) for 3 consecutive days before inducing skin inflammation.
7
Animal Experiments and Cell Culture Protocol
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C57BL/6 mice (male, five weeks old) were purchased from Orient Bio (Iksan, Korea) and raised in plastic cages with plenty of water and food. Guidelines from the Institutional Animal Care and Use Committee at Sungkyunkwan University were followed in this study (SKKUIACUC-2021-07-18-1).
LNCaP cells were cultured in RPMI 1640 medium containing 10% FBS and 1% antibiotics (penicillin and streptomycin) at 37 °C, and HDP cells were cultured in HDP growth medium at 37 °C. Both cell lines were incubated with 5% CO2.
LNCaP cells were cultured in RPMI 1640 medium containing 10% FBS and 1% antibiotics (penicillin and streptomycin) at 37 °C, and HDP cells were cultured in HDP growth medium at 37 °C. Both cell lines were incubated with 5% CO2.
8
C57BL/6 Mice Husbandry and Housing
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Thirty C57BL/6 mice were purchased from Orient Bio, Inc. at the age of eight weeks (Seongnam-si, Gyeonggi-Do, Republic of Korea) and housed in a semi-pathogen-free facility. No more than 5 mice were housed per cage and mice were freely provided with food and water under constant conditions of temperature (23 °C) and humidity (60%). All animal procedures were approved by the Chungbuk National University Animal Ethics Committee (CBNUA-873-15-02).
9
Immunosuppression in Rodent Models
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All experiments using mice and rats were conducted in accordance with a protocol approved by the Institutional Animal Care and Use Committee (IACUC) of the Catholic University (Seoul, Korea). Nonobese diabetic/severe combined immunodeficient (NOD/SCID)/IL-2Rγ-/- (NOG/SCID) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Female Sprague-Dawley rats and C57BL/6 mice were purchased from Orient Bio (Seongnam, Korea). Animals were housed under pathogen-free conditions and were given autoclaved food and water.
To establish immunosuppression in the Sprague-Dawley rats and C57BL/6 mice, FK-506 (tacrolimus) and dexamethasone (Cayman Chemical, Ann Arbor, MI, USA) were dissolved in saline at a concentration of 1 mg/mL and sterilized with a 0.22-µm filter (Millipore Co., Billerica, MA, USA). Both immunosuppressants were intraperitoneally administered 1 mg/kg/day each for 3 days before cell injection.
To establish immunosuppression in the Sprague-Dawley rats and C57BL/6 mice, FK-506 (tacrolimus) and dexamethasone (Cayman Chemical, Ann Arbor, MI, USA) were dissolved in saline at a concentration of 1 mg/mL and sterilized with a 0.22-µm filter (Millipore Co., Billerica, MA, USA). Both immunosuppressants were intraperitoneally administered 1 mg/kg/day each for 3 days before cell injection.
10
Beetroot Radioprotection in Mice
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C57BL/6 mice (Orientbio, Sungnam, Korea) were housed in conventional animal facilities with an NIH-07-approved diet and water ad libitum at a constant temperature (23 ± 3 °C) and humidity (50 ± 5%) according to the guidelines for the Care and Use of Laboratory Animals of the institutional Ethical Committee of Jeju National University. Mice were 24–30 g of weight and 10–15 weeks of age. Mice were randomly separated into three groups (3–4 mice/group): non-irradiated group (Naive), irradiated control group (IR) and irradiation plus beetroot-treated group (IR + Beetroot). Experiments were repeated three times with a minimum of three mice in each.
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