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Csu 10 scanner head

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The CSU-10 scanner head is a key component of Yokogawa's confocal microscopy system. It functions as a high-speed, multi-point confocal scanning unit, enabling rapid acquisition of confocal images.

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Lab products found in correlation

C10600 10b ccd camera, by Yokogawa (5 mentions) Spinning disk confocal system, by PerkinElmer (4 mentions) Ti u inverted microscope, by Nikon (4 mentions) Ofn25 60 objective, by Hamamatsu Photonics (2 mentions) Alexa 488 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions) Mouse anti ha, by Fortrea (1 mentions) Alexa 647 conjugated goat anti hrp, by Jackson ImmunoResearch (1 mentions) Mouse anti 1d4 fasii, by Developmental Studies Hybridoma Bank (1 mentions) Cyanine 3 conjugated goat anti rabbit, by Jackson ImmunoResearch (1 mentions)

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CSU-10 head (7 citations)

5 protocols using csu 10 scanner head

1

Fluorescent Embryo Imaging Protocol

Cited 1 time
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Dechorionated, formaldehyde-fixed, methanol-devittellinized embryos were fluorescently stained using standard methods (see Supplemental Experimental Procedures for a list of antibodies used). Embryos were mounted in 70% glycerol/PBS. Fluorescent mRNA in situ hybridization was performed as described, with digoxigenin labeled probe (Santiago et al., 2014 (link)). fra antisense probe was transcribed from linearized cDNA cloned into pBluescript. Fluorescence quantification was performed as described (Santiago et al., 2014 (link)). Images were acquired using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon OFN25 60× objective with a Hamamatsu C10600-10B CCD camera and Yokogawa CSU-10 scanner head with Volocity imaging software. Max projections were generated, cropped and processed using ImageJ.
Santiago C, & Bashaw G.J. (2017). Islet coordinately regulates motor axon guidance and dendrite targeting through the Frazzled/DCC receptor. Cell reports, 18(7), 1646-1659.
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2

Fluorescent Staining of Drosophila Embryos

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Dechorionated, formaldehyde-fixed, methanol devitellinized embryos were fluorescently stained as previously described (Bashaw, 2010b ). Live-dissected embryos were stained as previously described (Bashaw, 2010a ). The following primary antibodies were used: mouse anti-1D4/FasII [Developmental Studies Hybridoma Bank (DSHB); 1:100], mouse anti-Beta gal [DSHB; 1:150], mouse anti-Robo [DSHB; 1:50], mouse anti-Myc [DSHB (9E10); 1:500] rabbit anti-GFP [Invitrogen #A11122); 1:500], mouse mAb anti-V5 [Serotec; 1:200], Mouse anti-HA [Covance (16B12) 1:250], Mouse anti-Sema2a [DSHB 1:10], Alexa647-conjugated goat anti-HRP [1:500, Jackson Immunoresearch (#123-605-021); 1:500]. Cyanine 3-conjugated goat anti-rabbit [Jackson; 1:1000], Alexa488-conjugated goat anti-mouse [Molecular Probes; 1:500] were used as secondary antibodies. Images were acquired using a spinning disk confocal system (PerkinElmer) built on a Nikon Ti-U inverted microscope using a Nikon OFN25 60X or 40X objective with a Hamamatsu C10600-10B CCD camera and Yokogawa CSU-10 scanner head with Volocity imaging software. Images were processed using ImageJ.
Hernandez-Fleming M., Rohrbach E, & Bashaw G.J. (2017). Sema-1a reverse signaling promotes midline crossing in response to secreted Semaphorins. Cell reports, 18(1), 174-184.
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3

Embryo Fixation and Confocal Imaging

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Embryo fixation and staining were performed as described (Kidd et al., 1998 (link)). Images were acquired with Volocity using a spinning disk confocal (Perkin Elmer) using a Nikon 40x objective with a Hamamatsu C10600-10B CCD camera and Yokogawa CSU-10 scanner head. Images were processed using ImageJ.
Santiago C., Labrador J.P, & Bashaw G.J. (2014). The homeodomain transcription factor Hb9 controls axon guidance in Drosophila through the regulation of Robo receptors. Cell reports, 7(1), 153-165.
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4

Fluorescence Quantification in Drosophila Embryos

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Phenotypes were analyzed and images were acquired using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon OFN25 60x 40x or 10x objective with a Hamamatsu C10600-10B CCD camera and Yokogawa CSU-10 scanner head with Volocity imaging software. Images were processed using ImageJ and Adobe Illustrator software. For fluorescence quantification of GFP antibody staining in embryos, ten embryos per genotype (+/+; UAS-Apc2GFP or brat(-); UAS-Apc2GFP, +/+; src64bGFP or brat(-); scr64bGFP) were imaged using identical settings. Max projections were generated using ImageJ. After subtracting the staining background, the average pixel intensity was measured on twelve to sixteen clusters of EW neurons or across five regions within longitudinal axons for each embryo. The values from the five to ten embryos for each phenotype were averaged.
Arbeille E, & Bashaw G.J. (2018). Brain Tumor promotes axon growth across the midline through interactions with the microtubule stabilizing protein Apc2. PLoS Genetics, 14(4), e1007314.
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5

Embryo and S2R+ Cell Imaging Protocol

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Images of embryos and S2R+ cells were acquired using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon OFN25 60× objective with a Hamamatsu C10600-10B CCD camera and Yokogawa CSU-10 scanner head with Volocity imaging software. Images were processed using ImageJ. When scoring EW crossing, a segment was considered to have a crossing defect if one or both bundles of EW axons failed to reach the midline. When scoring ap crossing, a segment was considered to have an ectopic cross if it contained at least one continuous projection that extended across the midline and reached the lateral bundle of ap axons on the contralateral side. comm expression was scored using Volocity imaging software. Embryos expressed UAS-Tau-Myc-GFP and EW or ap neurons were identified by anti-Myc immunostaining. If the cell body of a neuron could be detected by the in situ signal, that neuron was scored as positive. Crossing and comm expression were scored in EW neurons at stage 14 and in ap neurons at stages 16-17. For all analyses, segments A1-A7 were scored. Midline crossing phenotypes and comm mRNA expression were scored blind to genotype whenever possible.
Neuhaus-Follini A, & Bashaw G.J. (2015). The intracellular domain of the Frazzled/DCC receptor is a transcription factor required for commissural axon guidance. Neuron, 87(4), 751-763.
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