After the cells were treated with 0, 1, 10, and 100 μM THSG in PluriSTEMTM ES/iPS Medium (Merk) for 3 h, protein extraction and Western blotting were performed as described previously.13 (link) The resolved proteins were then incubated with rabbit anti-JAK2, rabbit anti-phospho-JAK2, rabbit anti-STAT3, rabbit anti-phospho-STAT3 (Cell Signaling Technology, Beverly, MA, USA), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Merk, Darmstadt, Germany) at 4 °C overnight. Signals were detected using the LumiFlas™ Femto Chemiluminescent Substrate, HRP kit (ENERGENESIS BIOMEDICAL, Taipei, Taiwan). Images of the Western blots were visualized and analyzed using the Chemi-Doc™ XRS + System (Bio-Rad Laboratories, Hercules, CA, USA).
Rabbit anti phospho jak2
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-phospho-JAK2 is a primary antibody that recognizes the JAK2 protein when phosphorylated at specific amino acid residues. This product can be used to detect the phosphorylation state of JAK2 in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti stat3, by Cell Signaling Technology (2 mentions)
Rabbit anti phospho stat3, by Cell Signaling Technology (2 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
Rabbit anti jak2, by Cell Signaling Technology (1 mentions)
Rabbit anti mmp 2, by Cell Signaling Technology (1 mentions)
Mouse anti vimentin, by BD (1 mentions)
Rabbit anti twist, by Cell Signaling Technology (1 mentions)
Il 6 neutralizing antibody, by R&D Systems (1 mentions)
Recombinant human il 6, by R&D Systems (1 mentions)
Mouse anti α sma, by Abcam (1 mentions)
Stattic, by Merck Group (1 mentions)
Mouse anti e cadherin, by Thermo Fisher Scientific (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
2 protocols using rabbit anti phospho jak2
1
THSG Modulates JAK2/STAT3 Signaling
2
Investigating Cytokine and Signaling Pathways in EMT
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human recombinant IL-6 and IL-6 neutralizing antibody were purchased from R&D Systems Inc. (Minneapolis, MN, USA). STAT3 inhibitor Stattic was purchased from Sigma-Aldrich Inc. (St Louis, MO, USA), JAK2 inhibitor AG490 was purchased from Abcam (Cambridge, MA, USA).
Antibodies used for western blotting were mouse anti-E-cadherin (Invitrogen, Carlsbad, CA, USA), mouse anti-vimentin (BD Bioscience, San Jose, CA, USA), rabbit anti-phospho-JAK2 (Cell Signaling Technology, Beverly, MA, USA), rabbit anti-STAT3 (Cell Signaling Technology), rabbit anti-phospho-STAT3 (Cell Signaling Technology), mouse anti-α-SMA (Abcam), rabbit anti-MMP2 (Cell Signaling Technology), mouse anti-VEGF (Cell Signaling Technology), rabbit anti-TWIST (Cell Signaling Technology), mouse anti-β-actin (Sigma-Aldrich). The secondary antibodies coupled to HRP were purchased from ZSGB-BIO (Beijing, China)
Antibodies used for western blotting were mouse anti-E-cadherin (Invitrogen, Carlsbad, CA, USA), mouse anti-vimentin (BD Bioscience, San Jose, CA, USA), rabbit anti-phospho-JAK2 (Cell Signaling Technology, Beverly, MA, USA), rabbit anti-STAT3 (Cell Signaling Technology), rabbit anti-phospho-STAT3 (Cell Signaling Technology), mouse anti-α-SMA (Abcam), rabbit anti-MMP2 (Cell Signaling Technology), mouse anti-VEGF (Cell Signaling Technology), rabbit anti-TWIST (Cell Signaling Technology), mouse anti-β-actin (Sigma-Aldrich). The secondary antibodies coupled to HRP were purchased from ZSGB-BIO (Beijing, China)
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