All antibodies were purchased from BD PharMingen (San Diego CA): CD45.2-APC/Cy7 (clone 104), CD11b-FITC (clone M1/70), Gr-1-PerCP-Cy5.5 (clone RB6-8C5), Ly6G-PerCP/Cy5.5 (clone 1A8), Ly6C-PE/Cy7 (clone AL-21), F4/80 (APC; clone BM8), CD3-PerCP (clone 145-2C11), CD4-FITC (clone RM4-5), CD8-PE (clone 53-6.7). Isotype control Abs and unstained samples were included in the analyses. Cells were incubated (20 min at 4°C) in FACS buffer (PBS, 2% FCS, 2 mM EDTA) containing an anti-mouse Fc receptor blocking reagent (Miltenyi). Afterward, cells were stained with fluorochrome-conjugated antibodies against CD45, CD11b, Ly6G, Ly6C, F4/80, CD3, CD4, and CD8 for 30 min at 4°C. Cells were acquired either on a CantoII or a FACSCalibur (both BD Bioscience). Data were analyzed using FlowJo v10.0 software (Tree Star). Reported numbers were normalized for the weight of total hearts or 1 ml blood, yielding the number of respective cell fraction per mg tissue or per ml blood.
Anti mouse fc receptor blocking reagent
Manufactured by Miltenyi Biotec
Sourced in United States
The anti-mouse Fc receptor blocking reagent is a laboratory product designed to block the Fc receptors on mouse cells. It is used to prevent non-specific binding of antibodies to Fc receptors, which can interfere with experimental results. The reagent is intended to improve the specificity and reliability of immunological assays and procedures involving mouse cells or tissues.
Automatically generated - may contain errors
Lab products found in correlation
Lsrfortessa x 20, by BD (2 mentions)
Facs caliber, by BD (2 mentions)
Rbc lysis buffer, by Thermo Fisher Scientific (2 mentions)
Cell staining buffer, by BioLegend (2 mentions)
Ly6g 1a8 and cd11b m1 70 antibodies, by Thermo Fisher Scientific (2 mentions)
Facscalibur, by BD (1 mentions)
Canto 2, by BD (1 mentions)
3 protocols using anti mouse fc receptor blocking reagent
1
Multi-Marker Immune Cell Profiling
2
Quantifying Blood Neutrophil Count in Mice
3
Quantifying Blood Neutrophil Count in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For blood neutrophil count, blood was collected from both uninfected and infected WT and NLRP6 KO mice at 48 h post-infection. Briefly, 100 μl of blood was added to a 5 ml Polystyrene Round Bottom tube and RBCs were lysed using RBC lysis buffer (Gibco, USA). Cells were incubated for 20 min at 4°C in cell staining buffer (Biolegend, USA) containing anti-mouse Fc receptor blocking reagent (Miltenyi Biotec). Afterwards, cells were stained with Ly6G (1A8) and CD11b (M1/70) antibodies (eBioscience) for 30 min at 4°C. Appropriate Isotype-matched control antibodies were used in a similar manner. Unstained samples were included in the analyses for the compensation. The cells were fixed with 2% paraformaldehyde and analyzed using either FACS caliber or LSRFortessaX20 (BD). Data was analyzed using FlowJo v10.0 software.
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