All mouse lines used in this study were on a C57BL6 genetic background. CRMP4-KO (Khazaei et al., 2014 (link)) and their WT littermates were obtained by crossing CRMP4-heterozygous mice. Single and double CRMP4/Sema3E heterozygotes and their WT littermates were obtained by crossing CRMP4-heterozygote with Sema3E-heterozygote mice. Thy1-eYFP line H mice (Feng et al., 2000 (link)) were obtained from Jackson Labs (B6.Cg-Tg(Thy1-YFP) HJrs/J, RRID:
B6 cg tg thy1 yfp hjrs j
The B6.Cg-Tg(Thy1-YFP)HJrs/J is a transgenic mouse strain that expresses yellow fluorescent protein (YFP) under the control of the Thy1 promoter. The Thy1 gene encodes a cell surface glycoprotein that is expressed in a subset of neurons, allowing for the visualization of neuronal structures in this model.
Lab products found in correlation
17 protocols using b6 cg tg thy1 yfp hjrs j
Genetic Mouse Models for Neurodevelopment
All mouse lines used in this study were on a C57BL6 genetic background. CRMP4-KO (Khazaei et al., 2014 (link)) and their WT littermates were obtained by crossing CRMP4-heterozygous mice. Single and double CRMP4/Sema3E heterozygotes and their WT littermates were obtained by crossing CRMP4-heterozygote with Sema3E-heterozygote mice. Thy1-eYFP line H mice (Feng et al., 2000 (link)) were obtained from Jackson Labs (B6.Cg-Tg(Thy1-YFP) HJrs/J, RRID:
Organotypic Hippocampal Slice Culture from Thy1-YFP Mice
The cultures were maintained in a humidified atmosphere of 34°C for 18 days before beginning experiments. Culture medium, composed of 50% minimal essential medium of Hanks' salt, 25% Hanks' basal salt solution, and 25% heat-inactivated horse serum (all from Gibco), was renewed every 3–4 days.
Transgenic Mouse Model for ALS
In Vivo Spine Analysis of α-Synuclein Overexpression
For in vivo spine analysis studies, we utilized the Thy1-YFP mouse line, encoding the fluorescent molecule, YFP under the control of the Thy1 promoter: B6.Cg-Tg(Thy1-YFP)HJrs/J; stock number 3782 (Jackson Labs), and the Thy1-GFP line for confocal imaging studies: Tg(Thy1-GFP)MJrs/J; stock number 007788 (Jackson Labs). For Thy1-YFP, males and females were used and randomized to either control (n = 6) or α-Synuclein overexpression (n = 8) treatment groups prior to experiment onset. All mice were injected as mature adults, with age range for controls 39–70 weeks old; α-Synuclein overexpression range 40–73 weeks old. The Thy1-GFP mice contained 4 mice (3 m, 1F); age at injection ranged from 11 to 24 weeks old. Mice were housed in groups of 2–4 per cage, except when directed to singly house by the facility veterinarian due to fighting. In general, the cranial window and head bar did not prevent group housing.
Transgenic Mouse Experimental Protocol
Transgenic Mouse Model for ALS
Sarm1 Knockout Mouse Model
Generation and Maintenance of Transgenic Mice
Cyfip2 Conditional Knockout Mouse Model
Thy1-YFPH Mouse Model for Neuronal Tracing
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