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Cgs21680

Manufactured by Bio-Techne
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CGS21680 is a laboratory equipment product offered by Bio-Techne. It is a specialized device designed for scientific research and analysis purposes. The core function of this product is to [concise description of the equipment's core function, without interpretation or extrapolation].

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Lab products found in correlation

Zm241385, by Bio-Techne (18 mentions) Sch58261, by Bio-Techne (11 mentions) Adenosine, by Merck Group (9 mentions) Bay 60 6583, by Bio-Techne (6 mentions) Forskolin, by Merck Group (5 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Skf81297, by Bio-Techne (4 mentions) Forskolin, by Bio-Techne (4 mentions) Sch442416, by Bio-Techne (4 mentions) 3 isobutyl 1 methylxanthine, by Merck Group (3 mentions) Pd98059, by Merck Group (3 mentions) Mrs2179, by Merck Group (3 mentions) Mrs2365, by Merck Group (3 mentions) 2 chloro n6 cyclopentyladenosine ccpa 8 cyclopentyl 1 3 dipropylxanthine dpcpx 2 3 benzoyl 4 benzoyl atp bzatp, by Merck Group (3 mentions) Periodate oxidized atp oxatp, by Merck Group (3 mentions) Nf340, by Bio-Techne (3 mentions) Myr pki, by Enzo Life Sciences (3 mentions) 14 22 amide myristoylated, by Enzo Life Sciences (3 mentions) N6 ph camp, by Biolog (3 mentions) Recombinant human transforming growth factor β1 tgf β1, by Merck Group (3 mentions)

65 protocols using cgs21680

1

CGS21680 Administration in Mice

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The A2Ar agonist, CGS21680 (Tocris, Bristol, U.K.) in DMSO (27.7 mg/mL) was diluted in PBS to a final concentration of 0.05 mg/mL. Mice received CGS21680 at 0.5 mg/kg given i.p. once a day for three days.
Lee D.J., Preble J., Lee S., Foster C.S, & Taylor A.W. (2016). MC5r and A2Ar Deficiencies During Experimental Autoimmune Uveitis Identifies Distinct T cell Polarization Programs and a Biphasic Regulatory Response. Scientific Reports, 6, 37790.
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2

Neuromuscular Junction Pharmacology

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The following drugs were used: Thrombin from human plasma (purchased from Sigma-Aldrich, USA); human isoform of BDNF (purchased from Alomone Labs, Jerusalem, Israel); (±)-Vesamicol hydrochloride and Bafilomycin A1 as direct and indirect inhibitor of vesicular ACh transport, respectively; ANA12 as TrkB receptor antagonist, U73122 and U73343 as PLC inhibitor and its inactive analog, respectively; U0126 and U0124 as MEK1/2 inhibitor and its inactive analog, respectively; H-89 dihydrochloride as PKA inhibitor; ZM241385 as adenosine A2A receptor antagonist and CGS21680 as A2A receptor agonist (purchased from Tocris, Bio-Techne, Minneapolis, MN, USA). Thrombin and BDNF were dissolved in deionized water. Stock solutions of all other drugs were prepared in DMSO (Helicon, Moscow, Russia). The final concentrations of DMSO in the working solution did not exceed 0.01% (v/v). At this concentration, the solvent did not affect the parameters of spontaneous and evoked activity in mouse NMJs. All drugs were applied via bath perfusion system (0.5 mL/min).
Gaydukov A., Bogacheva P., Tarasova E., Molchanova A., Miteva A., Pravdivceva E, & Balezina O. (2019). Regulation of Acetylcholine Quantal Release by Coupled Thrombin/BDNF Signaling in Mouse Motor Synapses. Cells, 8(7), 762.
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3

Effortful Choice Paradigm in Rats

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In order to assess the role of D2-expressing medium spiny neurons in our effortful choice paradigm, a subset of rats were injected intraperitoneally with vehicle (5% DMSO) or the selective A2A agonist CGS 21680 (Bio-Techne, Abingdon, U.K.; 0.05 and 0.1 mg/kg) immediately prior to behavioral testing. Doses were given in escalating order (0, 0.05, 0.1 mg/kg) on 3 days separated by 2-day washout periods.
Thompson A.B., Gerson J., Stolyarova A., Bugarin A., Hart E.E., Jentsch J.D, & Izquierdo A. (2017). Steep effort discounting of a preferred reward over a freely-available option in prolonged methamphetamine withdrawal in male rats. Psychopharmacology, 234(18), 2697-2705.
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4

Lipoprotein and Cell Membrane Studies

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Cholesterol and Methyl-β-cyclodextrin (MβCD) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), Lipofectamine 2000 transfection reagent and Opti-MEM reduced serum media were from Invitrogen Life Technologies (Carlsbad, CA, USA). CGS21680 and NECA were obtained from Tocris (Bristol, UK), and [3H] CGS21680 and [3H]ZM241385 were obtained from American Radiolabeled Chemicals (St. Louis, MO, USA).
McGraw C., Koretz K.S., Oseid D., Lyman E, & Robinson A.S. (2022). Cholesterol Dependent Activity of the Adenosine A2A Receptor Is Modulated via the Cholesterol Consensus Motif. Molecules, 27(11), 3529.
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5

Binding Assays for Adenosine Receptors

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PSB1115 (4-(2,3,6,7-Tetrahydro-2,6-dioxo-1-propyl-1H-purin-8-yl)-benzenesulfonic acid), ZM241385 (4-(2-[7-Amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)phenol), BAY60–6583 (2-[[6-Amino-3,5-dicyano-4-[4-(cyclopropylmethoxy)phenyl]-2-pyridinyl]thio]-acetamide), and CGS21680 (4-[2-[[6-Amino-9-(N-ethyl-β-D-ribofuranuronamidosyl)-9H-purin-2-yl]amino]ethyl]benzenepropanoic acid hydrochloride) were purchased from Tocris Bioscience (Minneapolis, MN). Nitrobenzylthioinosine (NBTI), dipyridamole, αβ-methylene ADP, and 5’-deoxycoformycin and Ac-YVAD-cmk were purchased from Sigma Aldrich (St. Louis, MO).
Chambers E.D., White A., Vang A., Wang Z., Ayala A., Weng T., Blackburn M., Choudhary G., Rounds S, & Lu Q. (2019). Blockade of equilibrative nucleoside transporter 1/2 protects against pseudomonas aeruginosa-induced acute lung injury and NLRP3 inflammasome activation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(1), 1516-1531.
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6

Synaptosomal cAMP Accumulation Assay

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Synaptosomal cAMP accumulation was measured using the LANCE Ultra cAMP kit (PerkinElmer, Waltham, MA, USA) as previously described49 (link). In brief, total striatal synaptosomal membranes (0.5 μg) from GPR37+/+ and GPR37−/− mice were resuspended in stimulation buffer (HBSS 1X, 5 mM Hepes pH 7.4, 10 mM MgCl2, 0.1% BSA) and subsequently processed for cAMP accumulation. Thus, vehicle, forskolin (1 μM; Sigma-Aldrich) or CGS21680 (500 nM; Tocris Biosciences, Bristol, UK) were added for 30 min at 22 °C before the lysis and cAMP quantification in a POLARStar microplate reader (BMG Labtech, Durham, NC, USA). cAMP levels were calculated as previously described49 (link).
Morató X., Luján R., López-Cano M., Gandía J., Stagljar I., Watanabe M., Cunha R.A., Fernández-Dueñas V, & Ciruela F. (2017). The Parkinson’s disease-associated GPR37 receptor interacts with striatal adenosine A2A receptor controlling its cell surface expression and function in vivo. Scientific Reports, 7, 9452.
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7

Optogenetic Modulation of Hippocampal LTP

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The recording of the evoked field excitatory postsynaptic potentials (fEPSP) in the CA1 stratum radiatum upon stimulation of Schaffer fibers every 20-sec, were as previously described69 in hippocampal slices (400μm) prepared two weeks after transfection with AAV5-CaMKIIa-mCherry without or with optoA2AR in hippocampus. A high frequency stimulation (HFS) train (100Hz, 1-sec) was used to induce long-term potentiation (LTP). Light stimuli, applied immediately before HFS, consisted of 3000 light pulses (465nm, 50ms pulse width, ~3-5mW/mm2 power density) over 300-sec and the optic fiber was placed over the slice between the stimulation and recording electrodes. CGS21680 (30nM; Tocris) was added to the superfusion solution 20-min before HFS onwards.
Li P., Rial D., Canas P.M., Yoo J.H., Li W., Zhou X., Wang Y., van Westen G.J., Payen M.P., Augusto E., Gonçalves N., Tomé A.R., Li Z., Wu Z., Hou X., Zhou Y., IJzerman A.P., Boyden E.S., Cunha R.A., Qu J, & Chen J.F. (2015). Optogenetic activation of intracellular adenosine A2A receptor signaling in hippocampus is sufficient to trigger CREB phosphorylation and impair memory. Molecular psychiatry, 20(11), 1339-1349.
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8

Adenosine Receptor Binding Assay

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Cholesterol and Methyl-β-cyclodextrin (MβCD) were obtained from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS), Lipofectamine 2000 transfection reagent and Opti-MEM reduced serum media were from Invitrogen Life Technologies (Carlsbad, CA). CGS21680 and ZM241385 were obtained from Tocris (Bristol, UK), FITC-APEC was obtained from the NIMH synthesis program, http://nimh-repository.rti.org, NIMH Code: D-906, and [3H]NECA and [3ZM241385] were obtained from American Radiolabeled Chemicals (St. Louis, MO).
McGraw C., Yang L., Levental I., Lyman E, & Skaja-Robinson A. (2019). Membrane Cholesterol Depletion Reduces Downstream Signaling Activity of the Adenosine A2a Receptor. Biochimica et biophysica acta. Biomembranes, 1861(4), 760-767.
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9

Induction of Respiratory Tolerance in Mice

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OT‐I mice were systemically tolerized by repeated i.p. injection of 2 μg OVA257 peptide in 100 μL PBS, on days 0, 1, 2, 3, 4, and 7. For A2A receptor agonist treatment, CGS21680 (Tocris Bioscience, 100 mM in DMSO) was diluted in PBS and injected separately i.p. at 25 μg/mouse. Control animals were given diluent (DMSO in PBS) alone. In IL‐12 neutralization experiments, 0.5 mg anti‐IL‐12 (clone C17.8, BioXCell, West Lebanon, NH) or rat IgG control (Sigma) was injected i.p. in PBS on days 0, 2, and 4. For respiratory tolerance in C57BL/6 mice, animals were given 50 μg OVA (Grade V, Sigma) in PBS i.n. on days 0, 1, 2, and 3. Mice were killed on day 6 and bronchoalveolar lavage performed with 1 mL PBS. Lungs were excised, cut into pieces, and digested with collagenase (type IV, Sigma, 0.5 mg/mL in DMEM + 10% FCS) for 45 min before release of lung cells by pipetting.
Noble A., Mehta H., Lovell A., Papaioannou E, & Fairbanks L. (2016). IL‐12 and IL‐4 activate a CD39‐dependent intrinsic peripheral tolerance mechanism in CD8+ T cells. European Journal of Immunology, 46(6), 1438-1448.
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10

Neuromodulatory Receptor Ligands Protocol

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CA200645 was from HelloBio (Bristol, UK). ZM 241385 and CGS21680 were from Tocris (Bristol, UK).
Stoddart L.A., Kindon N.D., Otun O., Harwood C.R., Patera F., Veprintsev D.B., Woolard J., Briddon S.J., Franks H.A., Hill S.J, & Kellam B. (2020). Ligand-directed covalent labelling of a GPCR with a fluorescent tag in live cells. Communications Biology, 3, 722.
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