Apc anti mouse cd11c clone n418

Manufactured by BioLegend

APC anti-mouse CD11c (clone N418) is a fluorescent-labeled antibody that binds to the CD11c surface marker expressed on mouse dendritic cells. It can be used for the identification and analysis of dendritic cells in flow cytometry applications.

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Lab products found in correlation

Rabbit monoclonal antibody against, by Cell Signaling Technology (1 mentions) Ab2907, by Abcam (1 mentions) A2859, by Merck Group (1 mentions) Fty720, by Merck Group (1 mentions) Apc anti mouse ifn γ clone xmg1, by BioLegend (1 mentions) Fitc anti mouse cd3 clone 17a2, by BioLegend (1 mentions) Apc cy7 anti mouse cd3 clone 17a2, by BioLegend (1 mentions) Facscanto instrument, by BD (1 mentions) Rbc lysis buffer, by BioLegend (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Ique screener plus, by Intellicyt (1 mentions) Anti human cd34 fitc, by BD (1 mentions) Pe anti mouse gr 1 clone rb6 8c5, by BioLegend (1 mentions)

4 protocols using apc anti mouse cd11c clone n418

Antibody Panel for Cell Signaling

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Rabbit polyclonal antibody CALR (ab2907), rabbit monoclonal phosphoneoepitope-specific antibody against phospho-eIF2α (Ser 51) (ab32157, clone E90), mouse monoclonal antibody against β-actin (ab49900, clone AC-15) were purchased from Abcam (Cambridge, UK). Rabbit polyclonal antibody against HRI (sc-30143), mouse monoclonal antibody against PKR (sc-6282, clone B-10) were purchased from Santa-Cruz biotechnology. Rabbit monoclonal antibody against PERK (#3192, clone C33E10), rabbit polyclonal antibody against GCN2 (#3302), rabbit polyclonal antibody against eIF2α (#99 722 S), Rabbit monoclonal antibody against LC3B (#2775), rabbit polyclonal antibody against GFP (#2555) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody anti-PD-1 (BE0273, clone 29F.1A12) and rat IgG1 anti-horseradish peroxidase isotype control (BE0088, clone HRPN) were purchased from BioXcell (West Lebanon, NH, USA). Mouse monoclonal antibody against Atg5 (A2859) was purchased from Merck-Sigma Aldrich. Anti-rabbit Alexa fluor®−488 coupled secondary antibody came from Thermo Fisher Scientific (#A11034). APC anti-mouse CD11c (Clone N418) was purchased from Biolegend (117310).

Bezu L., Wu Chuang A., Sauvat A., Humeau J., Xie W., Cerrato G., Liu P., Zhao L., Zhang S., Le Naour J., Pol J., van Endert P., Kepp O., Barlesi F, & Kroemer G. (2022). Local anesthetics elicit immune-dependent anticancer effects. Journal for Immunotherapy of Cancer, 10(4), e004151.

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Monoclonal Antibody Purification and Immune Cell Analysis

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Hybridoma producing anti‐l‐selectin monoclonal antibody Mel‐14 was grown and the antibody purified according to standard procedures. FTY720 was purchased from Sigma‐Aldrich. TLR‐grade Re‐form LPS from E. coli serotype R515 was purchased from Enzo Life Sciences. The antibodies used were as follows: anti‐asialo GM1 (Functional Grade Purified, cat. no. 16‐6507; eBioscience); PE anti‐mouse CD49b (clone DX5, cat. no. 108908; Biolegend); APC anti‐mouse IFN‐γ (clone XMG1.2, cat. no. 505810; Biolegend); FITC anti‐mouse CD3 (clone 17A2, cat. no. 100204; Biolegend); APC anti‐mouse CD11c (clone N418, cat. no. 117310; Biolegend); APC/Cy7 anti‐mouse CD3 (clone 17A2, cat. no. 100222; Biolegend); purified anti‐mouse CD31 (clone MEC 13.3, cat. no. 102502; Biolegend); PE/Cy7 anti‐mouse CD45.2 (clone 104, cat. no. 109830; Biolegend); and anti‐IL‐18 polyclonal antibody (cat. no. sc‐7954‐Y; Santa Cruz Biotechnology).

Mingozzi F., Spreafico R., Gorletta T., Cigni C., Di Gioia M., Caccia M., Sironi L., Collini M., Soncini M., Rusconi M., von Andrian U.H., Chirico G., Zanoni I, & Granucci F. (2016). Prolonged contact with dendritic cells turns lymph node‐resident NK cells into anti‐tumor effectors. EMBO Molecular Medicine, 8(9), 1039-1051.

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Analyzing Nasal Dendritic Cell Uptake

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Six hours after intranasally administering 50 μg/mouse AF488-OVA and 100 μg/mouse pCA to mice, single-cell suspensions were obtained from nasal tissues to analyze the uptake of AF488-OVA by dendritic cells (DCs) in the nasal mucosa. The nasal tissues were homogenized in PBS and centrifuged. To obtain single-cell suspensions, red blood cells were lysed with RBC lysis buffer (BioLegend, San Diego, CA, USA). The cells were then washed with staining buffer (PBS containing 2% heat-inactivated fetal bovine serum (FBS; Biowest, Nuaillé, France) and 0.1% sodium azide) and blocked with anti-mouse CD16/CD32 (clone 2.4G2, Tonbo Biosciences, San Diego, CA, USA) for 20 min on ice to block Fc receptors. After being washed with staining buffer, cells were stained with PE/Cy7 anti-mouse CD45 (clone 30-F11, BioLegend) in combination with APC anti-mouse CD11c (clone N418, BioLegend) or an isotype control for 30 min on ice. Finally, the samples were analyzed using a FACSCanto instrument (BD Biosciences, San Jose, CA, USA).

Tada R., Nagai Y., Ogasawara M., Saito M., Ohshima A., Yamanaka D., Kunisawa J., Adachi Y, & Negishi Y. (2024). Polymeric Caffeic Acid Acts as an Antigen Delivery Carrier for Mucosal Vaccine Formulation by Forming a Complex with an Antigenic Protein. Vaccines, 12(5), 449.

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Immune Cell Phenotyping by Flow Cytometry

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The cells of interest were taken up at the final concentration of 1 x 10⁶ cells/ml in PBS (Life Technologies #14190) plus 2% Fetal bovine serum (FBS, PAN Biotech #P30-1502) and seeded (200 μl/well) into a 96-well plate (Thermo Fisher Scientific #249570). The plate was centrifuged 300g for 5 min at 4 °C, the cells were washed twice, and then incubated for 30 min at 4 °C with the respective antibodies. For NUP cells and NUP-MDSC: PerCP anti-mouse/human CD11b (clone M1/70, BioLegend #101229), FITC anti-mouse Ly6C (clone HK1.4, BioLegend #128006), APC anti-mouse CD11c (clone N418, BioLegend #117310), and PE anti-mouse Gr-1 (clone RB6-8C5, BioLegend #108407). For PMN-MDSC: PE anti-mouse/human CD11b (clone M1/70, BioLegend #101208) and APC anti-human CD15 (Biolegend #323008). For human CD34+ cells, FITC anti-human CD34 (BD #555821) was used. The cells were centrifuged and washed again twice in PBS plus FBS, and then data was acquired using iQue Screener Plus (IntelliCyt). Data analysis was done using FlowJo software (https://www.flowjo.com/).

Krumm J., Petrova E., Lechner S., Mergner J., Boehm H.H., Prestipino A., Steinbrunn D., Deline M.L., Koetzner L., Schindler C., Helming L., Fromme T., Klingenspor M., Hahne H., Pieck J.C, & Kuster B. (2023). High-Throughput Screening and Proteomic Characterization of Compounds Targeting Myeloid-Derived Suppressor Cells. Molecular & Cellular Proteomics : MCP, 22(9), 100632.

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