Mab1568

Manufactured by Merck Group

Sourced in Germany

MAB1568 is a laboratory equipment product manufactured by the Merck Group. It is designed to perform specific functions within the research and scientific community. The core function of this product is to provide precise and reliable measurements, though the intended use may vary depending on the needs of the user.

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Lab products found in correlation

Ab5054, by Merck Group (2 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Mab377, by Merck Group (1 mentions) Ab5541, by Merck Group (1 mentions) Homer1, by Synaptic Systems (1 mentions) Rab10, by Cell Signaling Technology (1 mentions) α synuclein, by BD (1 mentions) Ab51502, by Abcam (1 mentions) Ab230261, by Abcam (1 mentions) Ab76442, by Abcam (1 mentions) Ab19136, by Abcam (1 mentions) Ab237703, by Abcam (1 mentions) Ab241060, by Abcam (1 mentions) Ab51252, by Abcam (1 mentions) Sc 23948, by Santa Cruz Biotechnology (1 mentions) Vamp2, by Synaptic Systems (1 mentions) Sc 58774, by Santa Cruz Biotechnology (1 mentions) Lsm 710, by Zeiss (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Slowfade, by Thermo Fisher Scientific (1 mentions)

8 protocols using mab1568

Immunofluorescence Staining of Free-Floating Sections

Cited 1 time

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Primary antibodies for 40-μm free-floating sections were mouse anti-calretinin (MAB1568; Millipore, Burlington, MA) (40 (link)) and rabbit anti-c-Fos (226 003; Synaptic Systems, Göttingen, Germany) (41 (link)), both diluted 1:1000. Images were acquired with either a widefield or confocal microscope. Quantitative cell counting was performed blinded from confocal images.

Bauer J.P., Rader S.L., Joffe M.E., Kwon W., Quay J., Seanez L., Zhou C., Conn P.J, & Lewis A.S. (2021). Modeling Intrahippocampal Effects of Anterior Hippocampal Hyperactivity Relevant to Schizophrenia Using Chemogenetic Excitation of Long Axis–Projecting Mossy Cells in the Mouse Dentate Gyrus. Biological Psychiatry Global Open Science, 1(2), 101-111.

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Validation of Calretinin Antibodies

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Pre-adsorption and Western blots evaluations of polyclonal rabbit anti-calretinin (Millipore, AB5054) and monoclonal mouse anti-calretinin (Millipore, MAB1568) antibodies have been used to determine their specificity for calretinin (Baizer and Broussard, 2010 (link); Fuentes-Santamaria et al., 2005 (link)). We further validated the specificity of both antibodies in mouse using Western blot following standard protocols. In brief, P7 mouse brain lysates were run through 10% SDS-PAGE and then transferred to a PVDF membrane (Bio-Rad). The membrane was then incubated with primary antibody at 4°C overnight. Horseradish peroxidase conjugated secondary antibodies against either mouse or rabbit IgG (Amersham) were used at 1:5000. The immunoblot bands were visualized with chemiluminescence (ECL-SuperSignal West Pico Chemilunimescence Substrate, Pierce). The total protein loaded in each blot was visualized by staining using 2% Comassie blue solution. The rabbit polyclonal and the mouse monoclonal antibodies were used with 1:6000 and 1:2000 dilutions and each showed a single band of the expected molecular weight of 29kDa (Schwaller, 2009 (link)), indicating that they specifically recognized the appropriate protein.

Liu W, & Davis R.L. (2014). Calretinin and calbindin distribution patterns specify subpopulations of type I and type II spiral ganglion neurons in postnatal murine cochlea. The Journal of comparative neurology, 522(10), 2299-2318.

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Comprehensive Antibody Panel for Neuronal Characterization

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Rab10 (4262S, Cell Signaling Technology) for immunoblot, Rab10 (ab237703, Abcam) for immunofluorescence, pRab10 for immunoblot (ab230261, Abcam), pRab10 for immunofluorescence (ab241060, Abcam), Hsc70 (ab19136, Abcam), α-Tubulin (SC23948, Santa Cruz Biotechnology), parvalbumin, (NBP2-50036, NovusBio), NeuN (MAB377, Millipore), SATB2 (ab51502 Abcam), calretinin (MAB1568, Millipore), DARPP32 (MAB4230, R&D Systems), ChAT (NBP2-46620, NovusBio), DAT loop (6-8D6 Santa Cruz Biotechnology), tyrosine hydroxylase (TH) (ab76442, Abcam), CD68 (NBP2-33337SS, NovusBio), GFAP (AB5541, Millipore), Olig2 (MABN50 Millipore), KDEL receptor (sc-58774, Santa Cruz Biotechnology), TGN46 (MA3-063, ThermoFisher), LAMP1 (1D4B, DHSB), EEA1 (NBP2-36568, NovusBio), α-Synuclein (610786, BD Transduction Lab), Synuclein (ab51252, Abcam), VAMP2 (104 211, Synaptic Systems), Homer1 (160 006 Synaptic Systems), Rab8a (ab188574).

Singh V., Menard M.A., Serrano G.E., Beach T.G., Zhao H.T., Riley-DiPaolo A., Subrahmanian N., LaVoie M.J, & Volpicelli-Daley L.A. (2023). Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain. Acta Neuropathologica Communications, 11, 201.

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Immunostaining of Utricles for Hair Cell Markers

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Utricles were permeabilized and blocked for 2–3 h at room temperature in 0.5% PBS-Triton (PBS-T) and 10% normal donkey or goat serum (Vector Labs). Samples were incubated overnight at 4°C in primary antibodies diluted in 2% donkey/goat serum and PBS-T, followed by three rinses in PBS-T. Primary antibody labeling was detected using Alexa Fluor-conjugated secondary antibodies (1:400; Invitrogen) and/or DAPI (1:1000) also in 2% donkey/goat serum, PBS-T for 3 h. Finally, samples were washed three times with PBS and mounted in SlowFade (Life Technologies) for imaging. Images were taken using a Zeiss LSM 710 microscope at 40×/1.4 Oil DIC M27 and/or 20×/0.8 M27. The following primary antibodies and dilutions were used in this study; Ms α Calbindin2 at 1:200 (MAB1568; Millipore), Rb α Calbindin2 at 1:200 (AB5054; Millipore), Gt α Anxa4 at 1:200 (AF4146; R&D Systems), Ms α Pou4f3 at 1:200 (sc-81980; Santa Cruz Biotechnology), Rb α Myosin VIIA at 1:200 (25-6790; Proteus BioScience), Gt α Spp1 at 1:200 (AF808; R&D Systems), Gt α Ocomodulin at 1:200 (sc-7446; Santa Cruz Biotechnology), Rb α Tnc at 1:200 (AB19013; Millipore), Ms α Mapt at 1:200 (4019; Cell Signaling Technology).

McInturff S., Burns J.C, & Kelley M.W. (2018). Characterization of spatial and temporal development of Type I and Type II hair cells in the mouse utricle using new cell-type-specific markers. Biology Open, 7(11), bio038083.

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Quantifying Neuronal Activation using Immunostaining

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Primary antibodies for 40 μm free-floating sections were mouse anti-calretinin (MAB1568, Millipore, Burlington, MA (40 (link))) and rabbit anti-cFos (226 003, Synaptic Systems, Goettingen, Germany (41 (link))), both diluted 1:1000. Images were acquired with either a widefield or confocal microscope. Quantitative cell counting was performed blinded from confocal images.

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Immunohistochemical Labeling of Calretinin and cFos

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Primary antibodies for 40 μm free-floating sections were mouse anti-calretinin (MAB1568, Millipore, Burlington, MA (38) ) and rabbit anti-cFos (226 003, Synaptic Systems, Goettingen, Germany (39)), both diluted 1:1000. Widefield fluorescent images were acquired with an AF6000 LX fluorescent microscopy system (Leica, Buffalo Grove, IL) equipped with an HCX PL FLUOTAR 10x objective (NA = 0.30).
Confocal images were acquired with an LSM 710 META Inverted microscope (Zeiss, White Plains, NY) equipped with a 20x Plan-Apochromat objective (NA = 0.8). Cell counting was performed blinded from confocal images.

Bauer J.P., Rader S.L., Joffe M.E., Kwon W., Quay J., Seanez L., Zhou C., Conn P.J., & Lewis A.S. (2020). Modeling intrahippocampal effects of anterior hippocampal hyperactivity relevant to schizophrenia using chemogenetic excitation of long axis-projecting mossy cells in the mouse dentate gyrus.

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Immunohistochemical Markers for Neural Cells

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Mouse anti-8-oxo-dG (NS45.1, Japan Institute for the Control of Aging, Nikken Seil Co., Ltd., Fukuroi, Shizuoka, Japan), mouse anti-FOSB antibody (1:500, 5G4, Cell Signaling Technology), mouse anti-BrdU antibody (1:800, Roche), rabbit anti-NeuN antibody (1:1000, ABN-78, Millipore), rabbit anti-GAD67 antibody (1:150, sc-5602, Santa Cruz), goat anti-doublecortin (DCX) antibody (1:200, sc-8066, Santa Cruz), goat anti-SOX2 antibody (1:250, sc-17320, Santa Cruz), rabbit anti-DRD3 antibody (1:100, ab42114, Abcam), and mouse anti-calretinin antibody (1:2000, clone 6B8.2, MAB1568, Millipore) were used as the primary antibodies for immunohistochemistry and immunofluorescence.

Haruyama N., Sakumi K., Katogi A., Tsuchimoto D., De Luca G., Bignami M, & Nakabeppu Y. (2019). 8-Oxoguanine accumulation in aged female brain impairs neurogenesis in the dentate gyrus and major island of Calleja, causing sexually dimorphic phenotypes. Progress in neurobiology, 180.

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Immunostaining Protocol for Postnatal Mouse Brain

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Postnatal day 7 and 14 mice were transcardially perfused with PBS followed by 4% PFA in PBS and post-fixed by immersion in the same fixative overnight at 4 °C. Brains were cut in 20 µm slices for all experiments. Primary antibodies in this study include the following: goat anti-Cux1 (1 : 25; Sc-6327, Santa Cruz Biotechnologies, USA), rat anti-Ctip2 (1 : 200; ab18465, Abcam, UK), rabbit anti-Tbr1 (1 : 200; ab31940, Abcam), mouse anti-PV (1 : 500, MAB 1572, Millipore, USA), mouse anti-calretinin (1 : 1000, mab1568, Millipore) rat anti-SST (1 : 50, mab354, Millipore), mouse anti-GAD65 (1 : 20; Developmental Studies Hybridoma Bank, USA), rabbit anti-PSD95 (1 : 100; ab18258, Abcam), mouse anti-NeuN clone A60 (1 : 100, Millipore), rabbit anti-NeuN (1 : 100, Millipore), and rabbit anti-GFP (1 : 200; ab6556, Abcam). Specific secondary antibodies Alexa 488, 555, and 647 (A555, A647, and A488, Life Technologies, Belgium) were used.

Morelli G., Avila A., Ravanidis S., Aourz N., Neve R.L., Smolders I., Harvey R.J., Rigo J.M., Nguyen L, & Brône B. (2017). Cerebral Cortical Circuitry Formation Requires Functional Glycine Receptors. Cerebral cortex (New York, N.Y. : 1991), 27(3).

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