Clampex software

Manufactured by Molecular Devices

Sourced in United States

Clampex is a data acquisition software developed by Molecular Devices. It is designed to record and analyze electrophysiological data from various experimental setups, including patch-clamp recordings. Clampex provides a user-friendly interface for setting up and controlling the acquisition process, as well as tools for visualizing and analyzing the recorded data.

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Lab products found in correlation

Multiclamp 700b amplifier, by Molecular Devices (6 mentions) Axopatch 200b amplifier, by Molecular Devices (6 mentions) Clampfit, by Molecular Devices (5 mentions) Multiclamp 700b, by Molecular Devices (4 mentions) Digidata 1440, by Molecular Devices (4 mentions) Pclamp, by Molecular Devices (3 mentions) Clampfit software, by Molecular Devices (3 mentions) Digidata 1550b, by Molecular Devices (3 mentions) Axopatch 200b, by Molecular Devices (3 mentions) Digidata 1200, by Molecular Devices (2 mentions) Geneclamp 500b, by Molecular Devices (2 mentions) Originpro, by OriginLab (2 mentions) Spike2, by Cambridge Electronic Design (2 mentions) Igor pro, by Wavemetrics (2 mentions) Geneclamp 500b amplifier, by Molecular Devices (2 mentions) P 97 puller, by Sutter Instruments (2 mentions) Digidata 1440a, by Molecular Devices (2 mentions) Multiclamp 700b software, by Molecular Devices (2 mentions) Digidata 1322a digitizer, by Molecular Devices (2 mentions) Fura 2 am, by Thermo Fisher Scientific (2 mentions)

60 protocols using clampex software

Two-Electrode Voltage Clamp Protocols

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Two-electrode voltage clamp experiments were performed using GeneClamp 500B, DigiData 1200 or 1550 Interface and Clampex software (all Axon Instruments) at room temperature in ND96 extracellular media (in mM): NaCl 96, KCl 2, MgCl₂ 1, HEPES 5, CaCl₂ 1.8, pH 7.4. Recording electrodes were filled with 3 M KCl and had a tip resistance <1 MΩ. Data were filtered at 1 kHz and sampled at 5 kHz.
From a holding voltage of −80 mV, an activating pre-pulse step to +60 mV for 250 ms was applied before test voltage steps ranging from −150 to +190 mV in 10 mV increments for 250 ms, followed by a tail voltage step to −100 mV. For most cells the same protocol was also applied with a holding voltage of −40 mV as well as an additional protocol where the holding voltage was −80 mV and the pre-pulse step taken to −140 mV. These protocols are referred to as V_h = −40mV and V_pp = −140 mV, respectively.

Suetterlin K., Matthews E., Sud R., McCall S., Fialho D., Burge J., Jayaseelan D., Haworth A., Sweeney M.G., Kullmann D.M., Schorge S., Hanna M.G, & Männikkö R. (2021). Translating genetic and functional data into clinical practice: a series of 223 families with myotonia. Brain, 145(2), 607-620.

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Quantifying Cardiomyocyte Ca2+ Handling

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Sarcoplasmic reticulum (SR) Ca²⁺ load was calculated under the condition given in the previous section by the direct application 10 mM caffeine (in Ca²⁺ perfusion buffer for 20 s) immediately after deactivation of pacing using a separate gravity perfusion system attached to a heated (37°C) perfusion pencil (Digitimer). It was ensured that switching of pacing and caffeine application was instantaneous, by using Clampex software (Axon Instruments) to control each event. Fractional Ca²⁺ release from the SR was calculated by the division of [Ca²⁺]_i-transient amplitude preceding caffeine application by the SR [Ca²⁺]_i (given by the caffeine-transient amplitude). Sodium-calcium exchanger (NCX) activity was estimated by the τ-decay rate (τ₁) following 10-mM caffeine application. Sarcoendoplasmic reticulum ATPase (SERCA2a) activity was calculated by the subtraction of the fast [Ca²⁺]_i-transient τ₂ decay rate, which gives the total intracellular Ca²⁺ reuptake, from the slower τ₁ that was previously calculated (3 (link)).

Robinson P., Sparrow A.J., Patel S., Malinowska M., Reilly S.N., Zhang Y.H., Casadei B., Watkins H, & Redwood C. (2020). Dilated cardiomyopathy mutations in thin-filament regulatory proteins reduce contractility, suppress systolic Ca2+, and activate NFAT and Akt signaling. American Journal of Physiology - Heart and Circulatory Physiology, 319(2), H306-H319.

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Recording LTP in Mouse Hippocampal Slices

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Hippocampal slices (300 µm thick) were prepared from mouse brain, incubated at 32 °C for 30 min and maintained at 26 °C for 1 h as described previously.^[⁶⁴^] After recovery, slices were placed in a recording chamber at 25 °C and perfused with oxygenated artificial cerebrospinal fluid (ACSF) containing 126 mm
NaCl, 2.5 mm
KCl, 1.25 mm
NaH₂PO₄, 26 mm
NaHCO₃, 10 mm
glucose, 2 mm
CaCl₂, and 2 mm
MgSO₄ at a rate of 1 mL min⁻¹. Extracellular field EPSPs (fEPSPs) were recorded from the CA1 area using a glass electrode filled with ACSF (2–3 MΩ). Schaffer collateral pathway was stimulated every 30 s using concentric bipolar electrodes. A theta burst stimulation (TBS) protocol (four pulses of 100 Hz repeated three times at 5 Hz, and a 20 s inter‐train interval) was used to induce LTP.^[⁶⁴, ⁶⁵^] Field potentials were amplified, low‐pass filtered (MultiClamp 700B, Axon Instruments), digitized, and data were analyzed using the Clampex software (Axon Instruments).

Chen M., Zhang L., Shao M., Du J., Xiao Y., Zhang F., Zhang T., Li Y., Zhou Q., Liu K., Wang Z, & Wu B. (2022). E4BP4 Coordinates Circadian Control of Cognition in Delirium. Advanced Science, 9(23), 2200559.

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Electrophysiological Analysis of Neuron Activity

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All statistical values were expressed as mean ± standard error of the mean. The Shapiro-Wilk test was used to determine whether the data had a normal distribution. The unpaired t-test was used to compare the data from a normal distribution, while the Mann-Whitney test was used to compare data from a non-normal distribution. In addition, the percentage of the estrous cycle and pregnancy outcomes were analyzed using the chi-square test. Statistical analysis was performed in Origin software (OriginLab Corp, Northampton, MA, USA), and the tests performed are indicated in the figure legends. Statistical significance was defined as a p-value < 0.05. Acquisition and subsequent analysis of the acquired data were performed using Clampex software (Axon Instruments). The traces were plotted using Origin 8 software (OriginLab Corp., Northampton, MA, USA).

Bhattarai P., Rijal S., Bhattarai J.P., Cho D.H, & Han S.K. (2023). Suppression of neurotransmission on gonadotropin-releasing hormone neurons in letrozole-induced polycystic ovary syndrome: A mouse model. Frontiers in Endocrinology, 13, 1059255.

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Electrophysiological Data Acquisition

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Data were acquired using Clampex software (Axon Instruments), digitized at 35 to 50 kHz, and analyzed offline using pClamp (version 10.5, Axon Instruments), Spike2 (version 8.1, Cambridge Electronic Design), OriginPro (version 9.5, OriginLab Corporation), and SigmaPlot (version14, Systat Software) softwares.

Szegedi V., Bakos E., Furdan S., Kovács B.H., Varga D., Erdélyi M., Barzó P., Szücs A., Tamás G, & Lamsa K. (2023). HCN channels at the cell soma ensure the rapid electrical reactivity of fast-spiking interneurons in human neocortex. PLOS Biology, 21(2), e3002001.

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VDAC1 Reconstitution and Modulation

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VDAC1 was purified from rat liver mitochondria as described previously (Ben-Hail et al., 2016 (link)). To measure single and multiple channel current, a planar lipid bilayer (PLB) was prepared from soybean asolectin dissolved in n-decane (30 mg/ml). Purified VDAC1 (10–100 ng) was added to the chamber defined as the cis side containing 0.5 M NaCl. After one or a few channels were inserted into the PLB, excess protein was removed by perfusing the cis chamber with ∼10 volumes of solution to prevent further channel incorporation. Following several recordings of channel activity at different voltages, metformin or VBIT-4 was added to the cis chamber, and currents through the channel were again recorded. Currents were recorded by voltage-clamping using a Bilayer Clamp BC-535B amplifier (Warner Instruments, Hamden, CT). Currents were measured with respect to the trans side of the membrane (ground). The currents were low pass-filtered at 1 kHz and digitized online using a Digidata 1440-interface board and Clampex software (Axon Instruments, Union City, CA). Analysis was done using pClamp 10.2 software (Axon Instruments, Union City, CA), or excel (Microsoft).

Zhang E., Mohammed Al-Amily I., Mohammed S., Luan C., Asplund O., Ahmed M., Ye Y., Ben-Hail D., Soni A., Vishnu N., Bompada P., De Marinis Y., Groop L., Shoshan-Barmatz V., Renström E., Wollheim C.B, & Salehi A. (2019). Preserving Insulin Secretion in Diabetes by Inhibiting VDAC1 Overexpression and Surface Translocation in β Cells. Cell Metabolism, 29(1), 64-77.e6.

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Electroretinography in Drosophila Larvae

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ERG was recorded in 1-day old flies using the same methods as previously described (Fabian-Fine et al., 2003 (link); Williamson et al., 2010 (link)). Briefly, flies were glued on glass slides using Elmer’s non-toxic glue. Both the reference and recording electrodes were made of glass pipettes filled with 3 M KCl. The light stimulus was computer-controlled using white light-emitting diode system (MC1500; Schott), and was provided in 1-s pulses. The data was recorded using Clampex software (version 10.1; Axon Instruments) and measured and analyzed using Clampfit software (version 10.2; Axon Instruments).

Yusuff T., Chang Y.C., Sang T.K., Jackson G.R, & Chatterjee S. (2023). Codon-optimized TDP-43 mediates neurodegeneration in a Drosophila model of ALS/FTLD. Frontiers in Genetics, 14, 881638.

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Electrophysiological Characterization of TWIK1 Channels

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The stable expression of TWIK1 were cells cultured for 12–24 h after split and then whole-cell patch clamp recordings were performed. A PC505B amplifier (Warner Instrument Corporation) was used to voltage-clamp isolated cells. The electrodes had a resistance of 3–5 MΩ. We applied a voltage protocol that stimulated TWIK1 channels from −20 mV to −100 mV for 2 s, and then returned to −20 mV. Clampex software (Version 10.4, Axon Instruments) was used to generate the protocol. The recordings were carried out at room temperature (25 ± 1 °C) [33 (link)]. Clampfit (Molecular Devices, San Jose, CA, USA) was used to obtain the current traces and to analyze data. The intracellular solution contained 10 mM HEPES, 130 mM KASP, 5 mM EGTA, 5 mM MgCl₂, pH 7.4. The extracellular solution contained 37 mM NaCl, 100 mM NH4Cl, 4 mM KCl, 10 mM HEPES, 10 mM Glucose, 1 mM MgCl₂, 1.8 mM CaCl₂, pH 7.4.

Wang J., Liu H., Sun Z., Zou X., Zhang Z., Wei X., Pan L., Stalin A., Zhao W, & Chen Y. (2023). The Inhibitory Effect of Magnolol on the Human TWIK1 Channel Is Related to G229 and T225 Sites. Molecules, 28(19), 6815.

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Neuronal Electrophysiology Techniques

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Slices were transferred to the setup and perfused with ACSF (5ml/min at 30–31 ºC). Recording ACSF contained CNQX (10 µM), AP‐5 (50 µM), and Gabazine (3 µM). Patch pipettes (3–5MΩ) were filled with intracellular solution containing (mM): 111 K‐gluconate, 8 KCl, 10 HEPES, 4 Mg‐ATP, 0.4 Na‐GTP, 10 K‐phosphocreatine, 0.2 EGTA, and 0.37% biocytin (7.4 pH; 290 ± 10mOsm). Recordings were made using an Axopatch 700B amplifier and Clampex software (sampling: 50kHz; filtering: 30kHz; Axon Instruments, Union City, CA). After determination of resting membrane potential, cells were kept in current clamp at –65mV through steady current injection. Data were analyzed using a custom‐written script in Matlab (version R2012a; The MathWorks, Inc., Natick, MA). Action potentials (APs) were required to exceed a membrane potential threshold of –20mV and a speed threshold of 10mV/ms to be included in analysis. AP threshold was defined by determining the peak of the AP derivative, and measuring the membrane potential at which 10% of this maximum was reached. AP half‐width was calculated as the time difference between the two time points corresponding to 50% of the AP amplitude (AP peak/AP threshold). For most neurons, pyramidal identity was confirmed after recording by biocytin staining, using the avidin‐biotin‐peroxidase method.27

Dubey M., Brouwers E., Hamilton E.M., Stiedl O., Bugiani M., Koch H., Kole M.H., Boschert U., Wykes R.C., Mansvelder H.D., van der Knaap M.S, & Min R. (2018). Seizures and disturbed brain potassium dynamics in the leukodystrophy megalencephalic leukoencephalopathy with subcortical cysts. Annals of Neurology, 83(3), 636-649.

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Optic Nerve Electrophysiology in Mice

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Optic nerve recording of compound action potentials (CAPs) was performed as described previously [14 (link)]. Nerves were harvested at the same time following onset but before peak of disease. Four nerves were measured per group per each of three to four independent experiments. Briefly, mice were euthanized with CO₂ and optic nerves were dissected and incubated in artificial cerebrospinal fluid, containing (mM): NaCl 125, NaH₂PO₄ 1.25, glucose 25, NaHCO₃ 25, CaCl₂ 2.5, MgCl₂ 1.3, KCl 2.5 and saturated with 95% O₂/5% CO₂. Nerves were drawn into suction electrodes for stimulation and recording at 37 °C. Signals were amplified and acquired with a Digidata 1440A under Clampex software (Axon instruments). Analysis was performed offline using Clampfit. Amplitude and conduction velocity values for individual components of the CAP were derived by fitting with multiple Gaussians using Origin Pro [15 (link)]. Statistical analysis was performed with Microsoft Excel and Graphpad Prism.

Carbajal K.S., Mironova Y., Ulrich-Lewis J.T., Kulkarni D., Grifka-Walk H.M., Huber A.K., Shrager P., Giger R.J, & Segal B.M. (2015). T-helper cell diversity in experimental autoimmune encephalomyelitis and Multiple Sclerosis. Journal of immunology (Baltimore, Md. : 1950), 195(6), 2552-2559.

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