Ab54481

Manufactured by Proteintech

Ab54481 is a laboratory equipment product offered by Proteintech. It serves as a tool for researchers and scientists to conduct experiments and analyses. The core function of this product is to provide a specific capability required in a laboratory setting, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Ab45688, by Abcam (2 mentions) Ab124964, by Proteintech (2 mentions) Oil red o, by Merck Group (2 mentions) Ab92501, by Abcam (1 mentions) Bms309403, by R&D Systems (1 mentions) Sc 7382, by Santa Cruz Biotechnology (1 mentions) Sc393906, by Abcam (1 mentions)

2 protocols using ab54481

Adipogenesis Regulation and Signaling

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We used antibodies to FABP4 (Abcam, ab92501, WB 1:1000 dilution; IF 1:50 dilution), fibronectin (Abcam, ab45688, 1:5000 dilution), α-SMA (Abcam, ab124964, 1:10,000 dilution), collagen-I (Proteintech, 14695-1-AP, 1:1000 dilution), TGF-β1 (Proteintech, 21898-1-AP, 1:1000 dilution), p-Smad2 (CST, 3104 S, 1:1000 dilution), p-Smad3 (CST, 9520 T, 1:1000 dilution), PGC1α (Abcam, ab54481, 1:1000 dilution), PPARγ (Proteintech, 16643-1-AP, 1:1000 dilution), caspase-3 (Proteintech, 19677-1-AP, 1:1000 dilution), Bcl-2 (Santa Cruz, sc-7382, 1:1000 dilution), BIP (CST, 3177 S, 1:1000 dilution), CHOP (CST, 2895 T, 1:1000 dilution). Recombinant human TGF-β1 (Peprotech, 100-21C-10), Oil Red O (Sigma-Aldrich, O0625). FABP4 inhibitor BMS309403 (R&D systems, 5258/10).

Chen Y., Dai Y., Song K., Huang Y., Zhang L., Zhang C., Yan Q, & Gao H. (2021). Pre-emptive pharmacological inhibition of fatty acid–binding protein 4 attenuates kidney fibrosis by reprogramming tubular lipid metabolism. Cell Death & Disease, 12(6), 572.

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Molecular Insights into TGF-β1-Mediated Fibrosis

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Anti-FATP2 (Santa Cruz, sc-393906, WB, 1:2000), anti-Fibronectin (Abcam, ab45688, WB, 1:5000), anti-α-SMA (Abcam, ab124964, WB, 1:10000), anti-Collagen I (Proteintech, 14695-1-AP, WB, 1:1000), anti-TGF-β1 (Proteintech, 21898-1-AP, WB, 1:1000), anti-p-Smad2 (CST, 3104S, WB, 1:1000), anti-p-Smad3 (CST, 9520T, WB, 1:1000), anti-PGC1α (Abcam, ab54481, WB, 1:1000), anti-PPARγ (Proteintech, 16643-1-AP, WB, 1:1000), anti-caspase 3 (Proteintech, 19677-1-AP, WB, 1:1000), anti-Bcl-2 (Santa Cruz, sc-7382, WB, 1:1000), anti-BIP (CST, 3177 S, WB, 1:1000), anti-CHOP (CST, 2895 T, WB, 1:1000). Recombinant human TGF-β1 (Peprotech, 100-21C-10), Oil Red O (Sigma-Aldrich, O0625). FATP2 inhibitor lipofermata (ChemBridge, 5830995).

Chen Y., Yan Q., Lv M., Song K., Dai Y., Huang Y., Zhang L., Zhang C, & Gao H. (2020). Involvement of FATP2-mediated tubular lipid metabolic reprogramming in renal fibrogenesis. Cell Death & Disease, 11(11), 994.

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