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Retro puro vector

Manufactured by Oligoengine
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Sourced in United States

The Retro.puro vector is a lab equipment product designed for molecular biology applications. It functions as a viral vector system used for the delivery and expression of genetic material in target cells. The core function of the Retro.puro vector is to facilitate the introduction and integration of desired genetic sequences into the host cell genome.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PSUPER‐retro‐puro vector plasmid SUPER.retro.puro vector PSUPER.retro.puro vector PSUPERIOR.retro.puro vector PSUPER.retro.puro retroviral vector

3 protocols using retro puro vector

1

Stable and Transient STIM1 Knockdown

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Stable knockdown of STIM1 was performed using pSUPER.Retro.puro vector (Oligoengine) encoding small hairpin RNA. STIM1 sh1 and sh2 has been previously described2 (link). STIM1 sh3 is targeting the following sequence: 5′-AGAAGGAGCTAGAATCTCAC-3′. A vector encoding non-targeting control shRNA was previously described37 . Transient knockdown of STIM1 in CRC cancer cells were achieved through transfection of siRNA into cells using lipofectamine 2000 (Invitrogen, Inc). The siRNA for STIM1 or control siRNA was purchased from Invitrogen, Inc. Cells were seeded in 6 well plastic plates 1 day before applying siRNA.
Wang J.Y., Sun J., Huang M.Y., Wang Y.S., Hou M.F., Sun Y., He H., Krishna N., Chiu S.J., Lin S., Yang S, & Chang W.C. (2014). STIM1 Overexpression Promotes Colorectal Cancer Progression, Cell Motility and COX-2 Expression. Oncogene, 34(33), 4358-4367.
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2

Knockdown of PYK2 by shRNA

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RNA interference of PYK2 was performed using the pSUPER.Retro.puro vector (Oligoengine, Seattle, WA, USA). To efficiently knockdown PYK2, 2 shRNAs targeting 2 different regions of the PYK2 gene were used. The target sequences were TGCACTTGACAAGAAGTCC (PYK2 sh1) and ACCCAGAAACTGCTCAAC (PYK2 sh2).
Shen J., Yang J., Sang L., Sun R., Bai W., Wang C., Sun Y, & Sun J. (2021). PYK2 mediates the BRAF inhibitor (vermurafenib)-induced invadopodia formation and metastasis in melanomas. Cancer Biology & Medicine, 19(8), 1211-1223.
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3

Stable and Transient STIM1 Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stable knockdown of STIM1 was performed using pSUPER.Retro.puro vector (Oligoengine) encoding small hairpin RNA. STIM1 sh1 and sh2 has been previously described2 (link). STIM1 sh3 is targeting the following sequence: 5′-AGAAGGAGCTAGAATCTCAC-3′. A vector encoding non-targeting control shRNA was previously described37 . Transient knockdown of STIM1 in CRC cancer cells were achieved through transfection of siRNA into cells using lipofectamine 2000 (Invitrogen, Inc). The siRNA for STIM1 or control siRNA was purchased from Invitrogen, Inc. Cells were seeded in 6 well plastic plates 1 day before applying siRNA.
Wang J.Y., Sun J., Huang M.Y., Wang Y.S., Hou M.F., Sun Y., He H., Krishna N., Chiu S.J., Lin S., Yang S, & Chang W.C. (2014). STIM1 Overexpression Promotes Colorectal Cancer Progression, Cell Motility and COX-2 Expression. Oncogene, 34(33), 4358-4367.
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