Rabbit anti smyd3

After 3 h in culture on the microprinted PDMS layers, cells were fixed with 4% PFA at room temperature (RT) for 15 min and then permeabilized with 0.2% Triton X-100 in PBS for 5 min at RT and washed 3 times in PBS. The immunochemistry process was performed following these steps: the fixed cells were incubated for 30 min in a blocking solution (0.2% tween, 1% Bovine Serum Albumine BSA, 1% Foetal Bovine Serum FBS) at RT, then the primary antibody was incubated for 40 min, washed three times in PBS-tween 0.2%, the secondary was incubated for 30 min, washed three times with PBS. The nucleus was then labeled with 4′,6-diamidino-2-phenylindole (DAPI) for 30 min at RT and the actin filaments (F-actin) with SiR-actin (Spirochrome) at 100 nM in PBS overnight at 4 °C. The antibodies used for the immunochemistry were: mouse anti-Flag (Sigma Aldrich M2 F3165) 1/1000, rabbit anti-SMYD3 (Abcam ab187149) 1/300, rabbit anti-TAZ (Cell Signaling Technology 4883) 1/500, mouse anti-YAP (Santa Cruz Biotechnology 101199) 1/250, rabbit anti-Pan tri-methyl lysine (Cell Signaling Technology #14680) 1/1000, rabbit anti-Pan di-methyl lysine (Cell Signaling Technology #14117) 1/1000, DNase 1 Alexa Fluor 594 (Life Technologies) 1/1000, Rabbit anti-SETDB1 (Santa Cruz Biotechnology sc-66884) 1/200.