Cells were seeded at a density of 250,000 in 35mm MatTek dishes with glass cover bottom (MatTek Corp.) for 48 hours and treated subject to low calcium treatment as described above. FluorBrite DMEM (Life Technologies) supplemented with 10% FBS and OxyFluor (Oxyrase) at 1% (v/v) was used as calcium repletion medium to minimize photoxicity and photobleaching. Images were acquired on an inverted wide field Zeiss microscope (Zeiss AxioObserver.Z1) equipped with an incubator chamber (37 ºC and 5% CO2) was used. A 63X water immersion objective, NA 1.33 was used. QuantEM 512SC camera (Photometrics) was used with no binning. Time-lapse images were taken in GFP channel (Filter Set 38HE, item number 489038-9901-000, Carl Zeiss, Inc) at 5 minute intervals for 12 hours. DECMA-1 antibody (Sigma-Aldrich) was used at 100μg/mL following calcium repletion.
Decma 1 antibody
Manufactured by Merck Group
The DECMA-1 antibody is a laboratory research tool used for the detection and analysis of the DECMA-1 protein. It is a highly specific antibody that binds to the DECMA-1 protein, allowing for its identification and quantification in various biological samples. The core function of the DECMA-1 antibody is to provide a reliable and sensitive method for researchers to study the expression and distribution of the DECMA-1 protein in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Filter set 38 he, by Zeiss (1 mentions)
Axio observer z1, by Zeiss (1 mentions)
4 aminophenylmercuric acetate apma, by Merck Group (1 mentions)
U2os cell, by American Type Culture Collection (1 mentions)
Batimastat bb94, by Merck Group (1 mentions)
Mmp 2, by R&D Systems (1 mentions)
Compound e, by Thermo Fisher Scientific (1 mentions)
Recombinant dll4, by R&D Systems (1 mentions)
Mmp 9, by R&D Systems (1 mentions)
2 protocols using decma 1 antibody
1
Live-Cell Imaging of Calcium-Dependent Cell-Cell Adhesion
2
DLL4, MMP-2, and MMP-9 Regulation in U2OS Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Recombinant DLL4, MMP-2, and MMP-9 were purchased from R and D Systems. Batimastat (BB-94) was purchased from Sigma Aldrich. Compound E (GSI) was purchased from Fisher Scientific (Catalog # AAJ65131EXD). DECMA-1 antibody was purchased from Sigma-Aldrich (Cat# U3254, RRID:AB_477600 ). Z-DEV-FMK was purchased from R and D Systems (FMK004). E-cadherin primary antibody was purchased from Thermo Fisher Scientific (Cat# 33–4000, RRID:AB_2533118 ). β-tubulin primary antibody was purchased from Sigma-Aldrich (Cat#T8328, RRID:AB_1844090 ). U2OS cells were purchased from ATCC (Cat# HTB-96, RRID:CVCL_0042 ). MS5 and MS5-DLL4 cells were a kind gift from Dr. Stephen Blacklow. 4-aminophenylmercuric acetate (APMA) was purchased from Sigma-Aldrich. Herceptin was purchased from MedChemExpress (HY-P9907).
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