Myricetin

Manufactured by MedChemExpress

Sourced in China

Myricetin is a natural polyphenolic compound found in various plants. It serves as a laboratory reagent used for research and analytical purposes. Myricetin's core function is to act as a flavonoid and antioxidant in in vitro and in vivo studies.

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Lab products found in correlation

P38 mapk, by Abcam (1 mentions) Anti bcl 2, by Proteintech (1 mentions) March1, by Bioss Antibodies (1 mentions) Dimethyl sulfoxide (dmso), by Solarbio (1 mentions) Affinipure goat anti rabbit igg h l, by Proteintech (1 mentions) Stat3, by Proteintech (1 mentions) Gapdh, by Proteintech (1 mentions) Cyclin b1, by Proteintech (1 mentions) Cyclin d1, by Proteintech (1 mentions) P stat3, by Abcam (1 mentions) Mg132, by MedChemExpress (1 mentions) Baf a1, by MedChemExpress (1 mentions) Microplate reader, by Bio-Rad (1 mentions) Cck 8 reagent, by MedChemExpress (1 mentions)

3 protocols using myricetin

Myricetin modulates autophagy pathways

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Myricetin (#HY-15097) was purchased from MedChemExpress (New Jersey, United States), dissolved in dimethyl sulfoxide (DMSO, #D8370, Solarbio, Beijing, China) at a concentration of 100 mM, and stored at −20 °C. MG132 (#HY-13259) and BafA1 (#HY-100558) were also bought from MedChemExpress and dissolved in DMSO. All antibodies were as follows: anti-Bcl-2 (#12789-1-AP), Ki-67 (#27309-1-AP), Stat3 (#10253-2-AP), LC3 (#14600-1-AP), P62 (#18420-1-AP), CyclinB1 (#55004-1-AP), CyclinD1 (#26939-1-AP), GAPDH (#10494-1-AP), and peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H + L) (#SA00001-2) (Proteintech Group, Chicago, IL, United States); MARCH1 (#bs-9335R, Bioss, Beijing, China); p-Stat3 (#ab32143), p38 MAPK (#ab170099) (abcam, Cambridge, United Kingdom); p-p38 MAPK (#11581, Singalway Antibody, Maryland, United States); and MARCH1 (#YT2642, Immunoway, Newark, United States).

Yang W., Su J., Li M., Li T., Wang X., Zhao M, & Hu X. (2021). Myricetin Induces Autophagy and Cell Cycle Arrest of HCC by Inhibiting MARCH1-Regulated Stat3 and p38 MAPK Signaling Pathways. Frontiers in Pharmacology, 12, 709526.

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Myricetin Cytotoxicity Evaluation

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NCI-H446 and A549 cells were passaged in 6-well plates (4×10⁵ cells/well), incubated at 37°C in a 5% CO₂ cell incubator for 12h, and treated with different concentrations of myricetin (Cat#HY-15097, MedChemExpress). Meanwhile, a control group was set up. After 48 h of culture, the culture media were discarded, and the wells were washed three times with PBS. An amount of 600 μL 0.4% crystal violet (Cat#C0121, Beyotime Biotechnology) staining solution was added to each well and the staining solution was discarded after 10 min staining at room temperature. The wells were rinsed three times with PBS and conserved in a dry and cool environment for photographing and analysis.

Han J., Cheng C., Zhang J., Fang J., Yao W., Zhu Y., Xiu Z., Jin N., Lu H., Li X, & Li Y. (2022). Myricetin activates the Caspase-3/GSDME pathway via ER stress induction of pyroptosis in lung cancer cells. Frontiers in Pharmacology, 13, 959938.

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Evaluation of Neuroprotective Effects

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NPMSCs were detected by CCK-8 assays. Briefly, rat NPM-SCs were seeded onto 96-well plates (5 × 10 3 cells/well) at 37° with 5% CO 2 . When reaching 80-90% confluency, the cells were treated with 0-400 μM of H 2 O 2 for 0-6 h or 0-100 μM of myricetin (purity 98.08%; MedChemExpress, Shanghai, China) for 0-24 h followed by addition of 10 μL of CCK-8 reagent (MedChemExpress). After 4 h of incubation, the optical den-sity at 450 nm was estimated in a microplate reader (Bio-Rad, Hercules, CA, USA).

Xie T., Pan R., Huang W., Dong S., Wu S, & Ye Y. (2023). Myricetin alleviates H2O2-induced senescence and apoptosis in rat nucleus pulposus-derived mesenchymal stem cells. Folia histochemica et cytobiologica, 61(2).

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