Cetyltrimethylammonium chloride solution (CTAC, 25 wt%), tetraethyl orthosilicate (TEOS), triethanolamine (TEA), (3-aminopropyl)triethoxysilane (APTEOS), tacrolimus (Tac), ammonium nitrate (NH4NO3), dextran (Mw 9-11 000 g/mol, from Leuconostoc mesenteroides), 2-methoxypropene, and spermine were purchased from Sigma-Aldrich, while sulfo-Cy5.5 was purchased from Lumiprobe Corporation. All chemicals were used as received without further purification.
2 methoxypropene
Manufactured by Merck Group
Sourced in United States
2-methoxypropene is a colorless, volatile liquid chemical compound. It is used as a key intermediate in the synthesis of various organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Dextran, by Merck Group (7 mentions)
Anhydrous dimethyl sulfoxide, by Merck Group (5 mentions)
Triethylamine, by Merck Group (5 mentions)
Deuterium chloride, by Merck Group (3 mentions)
Deuterium oxide, by Thermo Fisher Scientific (3 mentions)
Tween 80, by Merck Group (3 mentions)
Pyridinium p toluenesulfonate, by Merck Group (3 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Methanol, by Merck Group (2 mentions)
Dichloromethane, by Merck Group (2 mentions)
D mannitol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Tetrahydrofuran, by Merck Group (2 mentions)
Spermine, by Merck Group (1 mentions)
Tacrolimus, by Merck Group (1 mentions)
Ammonium nitrate, by Merck Group (1 mentions)
Acetic acid solution, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Curcumin, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
7 protocols using 2 methoxypropene
1
Multifunctional Silica Nanoparticles for Tacrolimus Delivery
2
Dextran Functionalization and Characterization
3
Synthesis of Acetylated Dextran (AcDX)
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The synthesis procedure of AcDX was the same as described elsewhere54 (link). Briefly, dextran (1.00 g, molecular weight ~10,000 Da, Sigma-Aldrich, USA) was dissolved in anhydrous dimethyl sulfoxide (10 mL, Sigma-Aldrich, USA) and purged with dry N2. Afterward, pyridinium p-toluenesulfonate (15.6 mg, Sigma-Aldrich, USA) was added, followed by 2-methoxypropene (3.4 mL, Sigma-Aldrich, USA). The reaction was maintained at a slightly positive pressure of dry N2 for 4 h, during which the whole system was sealed to prevent the evaporation of 2-methoxypropene. The reaction was quenched with triethylamine (0.5 mL, Sigma-Aldrich, USA), and the reaction product was precipitated in water (100 mL, pH 8). After centrifugation (10 min, 20,000 × g), the resulting pellet was washed twice thoroughly with water (100 mL). Residual water was removed by freeze dryer for 48 h, yielding AcDX. The 1H NMR spectra (Supplementary Fig. S20 ) of bare dextran and AcDX were recorded at room temperature using Bruker Ascend spectrometer (400 MHz).
4
Synthesis of Acetalated Dextran Polymer
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Acetalated
dextran was synthesized according to the reaction reported elsewhere.12 (link),60 (link),61 (link) Briefly, we added dextran (1
g, MW 9000–11,000 kDa; Sigma-Aldrich, USA) to a two-neck flask,
previously dried, and we purged the flask with dry N2.
dextran powder was dissolved in anhydrous dimethyl sulfoxide (10 mL,
Sigma-Aldrich, USA) before adding pyridinium-p-toluenesulfonate
(15.6 mg; Sigma-Aldrich, USA) and 2-methoxypropene (3.4 mL; Sigma-Aldrich,
USA). We quenched the reaction with trimethylamine (1 mL; Sigma-Aldrich,
USA) after 1 h, and we employed H2O (200 mL) to precipitate
the modified dextran. The pellet was centrifuged (10 min, 20,000g) and washed twice with trimethylamine solution (100 mL;
0.01% v/v; pH 8). Finally, to remove the residual trimethylamine solution,
the powder was vacuum-dried at 40 °C for 48 h, producing a fine
white powder of acetalated dextran (1.10 g).15 (link)
dextran was synthesized according to the reaction reported elsewhere.12 (link),60 (link),61 (link) Briefly, we added dextran (1
g, MW 9000–11,000 kDa; Sigma-Aldrich, USA) to a two-neck flask,
previously dried, and we purged the flask with dry N2.
dextran powder was dissolved in anhydrous dimethyl sulfoxide (10 mL,
Sigma-Aldrich, USA) before adding pyridinium-p-toluenesulfonate
(15.6 mg; Sigma-Aldrich, USA) and 2-methoxypropene (3.4 mL; Sigma-Aldrich,
USA). We quenched the reaction with trimethylamine (1 mL; Sigma-Aldrich,
USA) after 1 h, and we employed H2O (200 mL) to precipitate
the modified dextran. The pellet was centrifuged (10 min, 20,000g) and washed twice with trimethylamine solution (100 mL;
0.01% v/v; pH 8). Finally, to remove the residual trimethylamine solution,
the powder was vacuum-dried at 40 °C for 48 h, producing a fine
white powder of acetalated dextran (1.10 g).15 (link)
5
Multifunctional Fluorescent Nanoparticles
6
Synthesis and Characterization of Dextran-mPEG Conjugates
7
Tacrolimus Cytotoxicity Assay in A549 Cells
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