Log In Sign up
The largest database of trusted experimental protocols

Ab66506

Manufactured by R&D Systems
Visit Supplier

Ab66506 is an antibody product developed by R&D Systems. It is a purified polyclonal antibody that recognizes a specific target protein. The antibody can be used for various laboratory applications, but a detailed description of its intended use or performance characteristics is not available.

Automatically generated - may contain errors

Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (4 mentions) Ab7842, by Abcam (2 mentions) Ab30477, by Abcam (2 mentions) Ab103790, by Abcam (2 mentions) Ab26405, by Abcam (2 mentions) Ab15160, by BioGenex (2 mentions) Ab20592, by Abcam (2 mentions) Ab10988, by Abcam (2 mentions) Af2419, by Abcam (2 mentions) Mu363 uc, by Abcam (2 mentions) Tsa cy3 kit, by Akoya Biosciences (2 mentions)

2 protocols using ab66506

1

Multiparametric Islet Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry of frozen or paraffin-embedded sections of pancreas, human islets, and human islet-like organoids (HILOs) was performed with antibodies to insulin (1/100, Abcam ab7842), c-peptide (1/100, Abcam ab30477), glucagon (1/100, Abcam ab10988), somatostatin (1/100, Abcam ab103790), pancreatic polypeptyde (1/100, Abcam, ab113694), NKX2–2 (1/100, DSHB, 74.5A5), NKX6–1 (1/100, DSHB, F55A12), MAFA (1/100, Abcam, ab26405), MAFB (1/100, Abcam, ab66506), PDX-1 (1/100, R&D, AF2419), CHGA (1/100, Abcam, ab15160), SYNAPTOPHYSIN (1/100, Biogenex, MU363-UC) and PD-L1 (1/100, Abcam, ab20592). For MAFA and MAFB, signal amplification was performed using TSA-Cy3 kit (Akoya Biosciences, SAT704A001EA). Secondary antibodies were coupled to Alexa 568, 647 (Life Technologies) and visualized by confocal microscopy (ZEISS) or fluorescence microscopy. Hoechst 33342 (Thermo Scientific, 62249, 1μg/ml final concentration) was used for nuclear staining.
Yoshihara E., O’Connor C., Gasser E., Wei Z., Oh T.G., Tseng T.W., Wang D., Cayabyab F., Dai Y., Yu R.T., Liddle C., Atkins A.R., Downes M, & Evans R.M. (2020). Immune evasive human islet-like organoids ameliorate diabetes. Nature, 586(7830), 606-611.
+ Open protocol
+ Expand
2

Multiparametric Islet Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry of frozen or paraffin-embedded sections of pancreas, human islets, and human islet-like organoids (HILOs) was performed with antibodies to insulin (1/100, Abcam ab7842), c-peptide (1/100, Abcam ab30477), glucagon (1/100, Abcam ab10988), somatostatin (1/100, Abcam ab103790), pancreatic polypeptyde (1/100, Abcam, ab113694), NKX2–2 (1/100, DSHB, 74.5A5), NKX6–1 (1/100, DSHB, F55A12), MAFA (1/100, Abcam, ab26405), MAFB (1/100, Abcam, ab66506), PDX-1 (1/100, R&D, AF2419), CHGA (1/100, Abcam, ab15160), SYNAPTOPHYSIN (1/100, Biogenex, MU363-UC) and PD-L1 (1/100, Abcam, ab20592). For MAFA and MAFB, signal amplification was performed using TSA-Cy3 kit (Akoya Biosciences, SAT704A001EA). Secondary antibodies were coupled to Alexa 568, 647 (Life Technologies) and visualized by confocal microscopy (ZEISS) or fluorescence microscopy. Hoechst 33342 (Thermo Scientific, 62249, 1μg/ml final concentration) was used for nuclear staining.
Yoshihara E., O’Connor C., Gasser E., Wei Z., Oh T.G., Tseng T.W., Wang D., Cayabyab F., Dai Y., Yu R.T., Liddle C., Atkins A.R., Downes M, & Evans R.M. (2020). Immune evasive human islet-like organoids ameliorate diabetes. Nature, 586(7830), 606-611.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!