Sepharose cl 4b

DTX was purchased from Wuhan Xinxin Jiali Biological Technology Co, Ltd (Wuhan, China). RGD, Lf, and coumarin-6 (C6, purity > 98%) were obtained from Sigma Aldrich (St. Louis, USA). Cholesterol (Chol) was purchased from Shanghai Xingzhi Chemical Factory Co, Ltd (Shanghai, China). Soya phosphatidyl choline (SPC) was obtained from Lipoid GmbH (Ludwigshafen, Germany). The DSPE-PEG2000 (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N- [methoxy(polyethyleneglycol)-2000]), DSPE-PEG₂₀₀₀-maleimide (DSPE-PEG₂₀₀₀-Mal), and DSPE-PEG₂₀₀₀-RGD were purchased from Ponsure Co, Ltd (Shanghai, China). 2-iminothiohne (Traut’s) was purchased from Thermo Fischer Scientific (Massachusetts, USA). Sephadex G-50, Sepharose CL-4B, Trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and TritonX-100 were purchased from Solarbio Co, Ltd (Beijing, China). Hoechst 33342 and DiR dye were purchased from YEASEN Co, Ltd (Shanghai, China). DMEM medium was obtained from Gibco (California, USA). Fetal bovine serum (FBS) was purchased from GEMINI (California, USA). The high-performance liquid chromatography (HPLC)-grade solvents used for HPLC were purchased from Xilong Chemical Co, Ltd. (Shantou, China). All other chemicals utilized were analytical grade preparations.

DTX was purchased from Wuhan XinxinJiali Biological Technology (Wuhan, China). Coumarin-6 (C6, purity > 98%; Sigma-Aldrich, St. Louis, USA), cholesterol (Xingzhi Chemical Factory, Shanghai, China), egg phosphatidylcholine (EPC; Lipoid GmbH, Ludwigshafen, Germany), DSPE-PEG2000 and DSPE-PEG2000-MAL (Ponsure, Shanghai, China) were sourced and used as supplied. Traut's reagent (2-iminothiohne) was purchased from Thermo Fisher Scientific (San Jose, CA, USA). Sephadex G-50, Sepharose CL-4B, trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) and TritonX-100 were purchased from Solarbio Technology (Beijing, China). Hoechst 33342, rhodaminephalloidin and DiR dyes were purchased from Yeasen Biotechnology (Shanghai, China). Dulbecco's modified Eagle's medium (DMEM; Gibco, Thermo Fisher Scientific, San Jose, CA, USA), fetal bovine serum (FBS; GeminiBio, West Sacramento, CA, USA) and high-performance liquid chromatography (HPLC) solvents (Xiqiao Chemical, Shantou, China) were among the chemicals purchased and used as acquired.
U87-MG glioma cells and immortalized hCMEC/D₃ were purchased from Guandao Biological Engineering (Shanghai, China) and when used, cultured in DMEM medium, supplemented with 10% FBS and 1% antibiotics (penicillin and streptomycin) under 5% CO₂ at 37ºC.