Human glioma cell lines U87MG, U373MG and GOS3 were obtained from ATCC (Rockville, MD) cultured in Dulbecco's Modified Eagle Medium (Sigma‐Aldrich, St Louis, MO) as described earlier.22 Normoxic (20% O2, 5% CO2, 75% N2) and hypoxic environment (0.2% O2, 5% CO2 and 94.8% N2) was created using AnoxomatJB (Drachten, Netherlands). For transfection of FAT1 and HIF1α siRNA, 2 × 105 cells were seeded per 25cm2 flask. After 24 hr, cells were transfected with siFAT1, siHIF1α and siControl according to the manufacturer's protocol with a final concentration of 100 nM of siRNA using Lipofectamine2000 and Opti‐Mem media (Invitrogen) and cultured under normoxic or hypoxic condition for 72 hr.
Gos 3
Manufactured by American Type Culture Collection
Sourced in United States
The GOS-3 is a lab equipment product designed for use in various scientific applications. It functions as a high-speed, high-capacity centrifuge, capable of efficient separation and concentration of biological samples. The GOS-3 offers reliable performance and consistent results, making it a valuable tool for researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
U87mg, by American Type Culture Collection (3 mentions)
U373mg, by American Type Culture Collection (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Merck Group (1 mentions)
Opti mem media, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Shg 44, by American Type Culture Collection (1 mentions)
Tj905, by American Type Culture Collection (1 mentions)
Svg p12, by American Type Culture Collection (1 mentions)
Astrocyte medium, by ScienCell (1 mentions)
3 protocols using gos 3
1
Hypoxic regulation of glioma cells
2
Culturing Human Brain Cell Lines
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Normal human brain astroglia cell line SVGP12, normal human astrocyte (HA), glioma cell line TJ905, GOS-3, U87MG, and SHG-44 were obtained from ATCC (Manassas, VA, USA). SVGP12 and U87 were cultured in EMEM (Hyclone, USA). HA was cultured in Astrocyte Medium (ScienCell, USA). GOS-3 and TJ905 were cultured in DMEM (Hyclone, USA). SHG-44 was cultured in RPMI 1640 (Hyclone, USA) [27 (link)]. All cells were cultured in a medium supplemented with 10% FBS (Gibco, USA) at 37°C incubation with 5% CO2.
3
Culturing Human Glioblastoma Cell Lines
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