568 α mouse secondary antibody

QM9 cells, seeded with coverslips in 12 well plates, were infected with rNDV and rNDV_G_RABV at an moi of 0.1, fixed with 4% paraformaldehyde 24 h p. i., and permeabilized using 0.1% Triton X-100. After blocking of permeabilized and non-permeabilized cells with 5% bovine serum albumin (BSA) in PBS, they were incubated with α NDV-HN and mAb RABV-G. Binding of primary antibody was visualized using Alexa 488 α-rabbit or 568 α-mouse secondary antibody (Invitrogen). 4’,6-Diamidino-2-phenylindole (DAPI) (Roth, Karlsruhe, Germany) was included in washing steps after binding of secondary antibodies to stain nuclei. Images were taken on a Leica SP5 confocal microscope (Leica Microsystems GmbH, Wetzlar, Germany) with an oil immersion objective (HCX PL APO 63x/1.40–0.60 objective). Sequential z-sections of stained cells were acquired for maximum projection, and images were processed using ImageJ software [42 (link)].

QM9 cells were infected with rNDV and rNDV_H_Kur at an moi of 0.1, fixed with 3.7% formaldehyde 24 h p.i., and permeabilized using 0.1% Triton X-100. After blocking of the cells with 5% bovine serum albumin (BSA) in PBS, they were incubated with α PPRV-H and monoclonal antibody (mAb) NDV-HN. Binding of primary antibody was visualized using Alexa 488 α-rabbit or 568 α-mouse secondary antibody (Invitrogen). 4′,6-Diamidino-2-phenylindole (DAPI) (Roth, Karlsruhe, Germany) was included in washing steps after binding of secondary antibodies to stain nuclei. Images were taken on a Leica SP5 confocal microscope (Leica Microsystems GmbH, Wetzlar, Germany) with an oil immersion objective (HCX PL APO 63×/1.40–0.60 objective). Sequential z-sections of stained cells were acquired for maximum projection, and images were processed using ImageJ software (Wayne Rasband, National Institutes of Health, USA).