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Inova 500 mhz 1h spectrometer

Manufactured by Agilent Technologies
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The Inova 500 MHz (1H) spectrometer is a nuclear magnetic resonance (NMR) spectrometer with a 500 MHz proton (1H) frequency. It is designed to perform high-resolution NMR analysis of samples.

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Lab products found in correlation

Z gradient 3 mm probe, by Agilent Technologies (2 mentions) 600 mhz 1h spectrometer, by Agilent Technologies (2 mentions) 5 mm z gradient probe, by Agilent Technologies (2 mentions) 3 mm gradient probe, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Unity INOVA 500 MHz (1H) spectrometer Inova 500 MHz spectrometer Inova 500 MHz Inova 500 spectrometer 500 MHz spectrometer Inova 500 Inova spectrometer 500 spectrometers Spectrometers

3 protocols using inova 500 mhz 1h spectrometer

1

Structural Characterization of NulO Pathway

Cited 1 time
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For structural characterization of NulO pathway intermediates, filtered enzymatic reactions (Amicon Ultra-4 centrifugal filter unit with 3000 kDa molecular weight cut-off) or material purified according to previously described methods was exchanged into 100% D2O (Gulati et al. 2015 (link)). Structural analysis was performed using either a Varian Inova 500 MHz (1H) spectrometer with a Varian Z-gradient 3-mm probe or a Varian 600 MHz (1H) spectrometer with a Varian 5 mm Z-gradient probe. All spectra were referenced to an internal acetone standard (δH 2.225 ppm and δC 31.07 ppm).
Friedrich V., Janesch B., Windwarder M., Maresch D., Braun M.L., Megson Z.A., Vinogradov E., Goneau M.F., Sharma A., Altmann F., Messner P., Schoenhofen I.C, & Schäffer C. (2017). Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications. Glycobiology, 27(4), 342-357.
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2

NMR Spectroscopy Characterization Protocol

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NMR experiments were carried out on a Varian INOVA 500 MHz (1H) spectrometer with 3 mm gradient probe at 25–50°C with acetone internal reference (2.225 ppm for 1H and 31.45 ppm for 13C), using standard pulse sequences gCOSY (gradient COrrelation SpectroscopY), TOCSY (Total Correlation Spectroscopy) (mixing time 120 ms), NOESY (Nuclear Overhauser Effect Spectroscopy) (mixing time 300 ms), gHSQC (gradient Heteronuclear Single Quantum Coherence), and gHMBC (gradient Heteronuclear Multiple Bond Coherence) (100 ms long range transfer delay). AQ time was kept at 0.8–1 sec for H-H correlations and 0.25 sec for HSQC. 256 increments were acquired for t1 in all 2D spectra, except 512 for gCOSY.
Yildiz F., Fong J., Sadovskaya I., Grard T, & Vinogradov E. (2014). Structural Characterization of the Extracellular Polysaccharide from Vibrio cholerae O1 El-Tor. PLoS ONE, 9(1), e86751.
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3

Structural Characterization of NulO Pathway

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For structural characterization of NulO pathway intermediates, filtered enzymatic reactions (Amicon Ultra-4 centrifugal filter unit with 3000 kDa molecular weight cut-off) or material purified according to previously described methods was exchanged into 100% D2O (Gulati et al. 2015 (link)). Structural analysis was performed using either a Varian Inova 500 MHz (1H) spectrometer with a Varian Z-gradient 3-mm probe or a Varian 600 MHz (1H) spectrometer with a Varian 5 mm Z-gradient probe. All spectra were referenced to an internal acetone standard (δH 2.225 ppm and δC 31.07 ppm).
Friedrich V., Janesch B., Windwarder M., Maresch D., Braun M.L., Megson Z.A., Vinogradov E., Goneau M.F., Sharma A., Altmann F., Messner P., Schoenhofen I.C, & Schäffer C. (2017). Tannerella forsythia strains display different cell-surface nonulosonic acids: biosynthetic pathway characterization and first insight into biological implications. Glycobiology, 27(4), 342-357.
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