Atat1

Antibodies used in this study: ATAT1 (Abcam, ab58742); LC3B (Sigma, L7543); HDAC6 (Cell Signaling, #7558); JNK1/p-JNK (Cell Signaling, #9252, #4668); Bcl-2/p-Bcl2 (Cell Signaling, #2870, #2827); LAMP1 (Abcam, ab24170); S6K, pS6K (Cell Signaling, #9202, #9205); 4E-BP1, p4E-BP1 (Cell Signaling, #9644, #2855). For immunoblotting, primary antibodies were used in 1:1,000 dilution, and for immunofluorescence imaging as 1:100-1:200 dilution, unless specified differently. Secondary antibodies were at 1:1,000 dilution. Un-cropped blots are shown in Supplementary Figs 9–13.

Whole-cell lysates were collected in the Laemelli sample buffer as previously described [18 (link)]; nuclear and cytoplasmic fractions were isolated as previously described [19 (link)]. Equal volumes of lysate were blotted and quantified as previously described [2 (link),18 (link),19 (link),20 (link)]. Three pairs of lysates were used to determine the levels of detyrosinated α-tubulin (Abcam, Cambridge, UK), HDAC6, ATAT1 (Abcam), SIRT2 (CST), TGM2 (CST), and HSP90α (CST) expression levels. Whole-cell lysates were collected from BoM-MDA-231 cells following growth under standard culture conditions and blotted. For all western blots conducted using cytoplasmic and whole-cell lysate, β-actin (CST) was used as a loading control. Lamin B1 (Santa Cruz Biotechnology, Dallas, TX, USA) was utilized as a loading control for western blots involving nuclear protein fractions.