For the ELISA, 5 × 104 mouse ASPS cells were seeded and cultured overnight. The culture media were collected and immediately analyzed using the mouse Gpnmb (Abcam), Vwf (Abcam), and Pdgfb (Proteintech) ELISA kits according to manufacturers’ instructions.
Pdgfb
Manufactured by Proteintech
Sourced in Israel
Pdgfb is a laboratory reagent used for research purposes. It is a growth factor that plays a role in cell proliferation, survival, and migration. Pdgfb is commonly used in cell culture experiments to study signaling pathways and cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Collagen 3, by Proteintech (1 mentions)
β actin, by Proteintech (1 mentions)
β tubulin, by Proteintech (1 mentions)
Dickkopf 1 dkk 1, by R&D Systems (1 mentions)
β catenin, by Proteintech (1 mentions)
Tnf α, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
2 protocols using pdgfb
1
ELISA Analysis of ASPS Cells
2
Citrus Peel Extract Modulates Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The dried citrus peel was purchased from the Jiangsu Provincial Hospital of Traditional Chinese Medicine (Nanjing, China). The CAE was then prepared as previously described. Dickkopf-1 (DKK1) was purchased from R&D Systems (USA); dimethyl sulfoxide, fetal bovine serum, Dulbecco’s modified Eagle’s medium and trypsin were purchased from Gibco, Thermo Fisher Scientific-CN (Shanghai, China); penicillin and streptomycin were purchased from Biological Industries (HaEmek, Israel); platelet-derived growth factor a (PDGF-a), PDGF-b, tumor necrosis factor α (TNF-α), matrix metalloproteinases-7 (MMP-7), CyclinD1, connective tissue growth factor (CTGF), α-smooth muscle aorta (a-SMA), collagen I, collagen III, β-catenin, glycogen synthase kinase-3β (GSK-3β), β-actin, β-tubulin, and GAPDH were purchased from Proteintech (Wuhan, China); and p53, p21, and p16 were purchased from Abcam (Cambridge, UK).
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