Enhanced chemiluminescence ecl western blotting chemiluminescent substrate

Phosphate-buffered saline (PBS) was purchased from Bio Basic Inc. (Markham, Ontario, Canada). 2,3,5-Triphenyl-tetrazolium chloride (TTC), cresyl violet, Evans blue (EB), and propidium iodide (PI) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The optimal cutting temperature compound cryostat embedding medium was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Primary antibodies against B-cell lymphoma 2 (Bcl-2), bcl-2-like protein 4 (Bax), phospho-phosphoinositide 3-kinase (p-PI3K), protein kinase B (PKB, AKT), phospho-extracellular signal-regulated kinase (p-ERK), ERK, phospho-c-Jun N-terminal kinase (p-JNK), JNK, phospho-p38 mitogen-activated protein kinase (p-p38), p38, and manganese superoxide dismutase (MnSOD) were purchased from Cell Signalling Technology (Danvers, MA, USA). PI3K, p-AKT, phospho-mammalian target of rapamycin (p-mTOR), sirtuin 1 (SIRT1), and β-actin were obtained from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Aquaporin 4 (AQP4) was purchased from Abcam Inc. (Milton, Cambridge, UK). The bovine serum albumin (BSA) standard and enhanced chemiluminescence (ECL) western blotting chemiluminescent substrate were purchased from Thermo Fisher Scientific.

Saline was from JW Pharmaceutical Co., Ltd. (Seoul, Korea); methanol from SK chemicals (Ulsan, Korea); cresyl violet (CV) and 2,3,5-triphenyl-tetrazolium chloride from Sigma-Aldrich Co. (St. Louis, MO, USA); and phosphate-buffered saline (PBS) from Bio Basic Inc. (Markham, Ontario, Canada). The optimal cutting temperature (OCT) compound cryostat embedding medium was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The primary antibodies against c-Jun N-terminal kinase (JNK), phospho-JNK (p-JNK), protein kinase B (Akt), p-Akt, manganese superoxide dismutase (Mn-SOD), and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). The secondary antibodies, goat anti-rabbit IgG pAb and goat anti-mouse IgG pAb, were obtained from Enzo Life Sciences Inc. (Farmingdale, NY, USA). Protein extraction solution was purchased from iNtRON (Seongnam-si, Gyeonggi-do, Korea). The BCA reagent, bovine serum albumin (BSA) standard, and enhanced chemiluminescence (ECL) western blotting chemiluminescent substrate were from Thermo Fisher Scientific (Waltham, MA, USA).