Antibodies used for western blotting include GAD1/2, EphA2, PDGFR (Cellsignal®), E-cadherin (BD Biosciences), Vimentin (SantaCruz), OVOL2, CBFA2T2, SOX2, ASCL1, POU5F1, GAPDH, SOX2, FYN, beta-actin (Sigma®). Fluorophore-conjugated primary antibodies were used for flow cytometry – CD56 BV605, CD151 PE, CD24 BUV395 (BD Biosciences), CD44 Pacific blue, E-cadherin Pacific blue (Cellsignal®), CADM1 A647 (MBL), EPHA2 A488 (R&D Systems), CD133 PE-Cy7 (Biolegend), Vimentin (Santacruz);
Cd151 pe
Manufactured by BD
CD151 PE is a fluorescently-labeled antibody that binds to the CD151 protein. CD151 is a member of the tetraspanin family of proteins, which are involved in various cellular processes. The CD151 PE antibody can be used in flow cytometry applications to identify and quantify cells expressing the CD151 protein.
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Lab products found in correlation
2 protocols using cd151 pe
1
Multiplex Protein Expression Analysis
2
Flow Cytometry Immunophenotyping Protocol
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For surface expression, cells were labeled with antibody or the corresponding isotype control in 1%BSA/PBS for 30 mins on ice. For total expression, cells were fixed with 4% paraformaldehyde and blocked with 1%BSA/PBS/0.2%Tween for 1 hr before labeling. Cells were washed 3 times and analyzed using an Acuri C6 flow cytometer; histograms were generated using FlowJo software. Mean fluorescence values were normalized to the “control” cell line level. Antibodies used were CD82-647 (Biolegend, ASL-24), CD81-FITC (Biolegend, 5A6), CD151-PE (BD Biosciences, 14A2.H1), and CD9-647 (Bio-Rad, MM2/57).
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